ISSN 1995-5537
"Biotechnology" journal Vol. 3, No. 5, 2010
Р. 57-65, Bibliography 18, Ukrainian.
Universal Decimal classification: 577.112.7:616
University of Kansas Medical Center, Kansas City, Kansas, USA;
Kyiv Taras Shevchenko National University;
Palladin Institute of Biochemistry of National Academy of Science of Ukraine, Kyiv
Transcription factor ZXDC participates in the regulation of MHCII gene expression in eukaryotes. Recently, using micro array and polymerase chain reaction assays, it was shown that overexpression of factor ZXDC in embryonic kidney cell line HEK293 enhances the expression of many genes, among them BDNF, CDKN1C, IL5Ra and EGR2. In this investigation, using quantitative polymerase chain reaction, it was shown that EGR2 mRNA expression is increased 2.3 times in embryonic kidney cell line HEK293 and almost 7 times in culture of myelocytes HL60 line which overexpress transcription factor ZXDC. In order to understand the induction mechanism of EGR2 gene expression, we cloned the promoter region of EGR2 gene (from –341 to +134) into a pGL3basic reporter plasmid with luciferase expression. It was determined that during co-transfection of HEK293 cell line with pGL-EGR2-341 and pcDNA3.1-ZXDC plasmid constructs, the expression of luciferase increased almost twice. Moreover, using chromatin immune precipitation assay, it was shown that the transcription factor ZXDC binds with the promoter region of EGR2 gene in promyelocytic leukemia cell line HL60 in vivo. The obtained data show that the transcription factor ZXDC regulates EGR2 gene transcription by binding with its promoter region.
Key words: transcription factors, ZXDC, EGR2, gene overexpression, transcription regulation, promyelocytic cells HL60, kidney cells HEK293.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008