ISSN 1995-5537
"Biotechnology" journal Vol. 3, No. 5, 2010
100-105, Bibliography 9, Russian.
Universal Decimal classification: 636.082.453.5
OPTIMIZATION OF CRYOPRESERVATION OF MOUSE EMBRYOS
L. V. Gorbunov, A. S. Salina, N. F. Kleshchev
National technical university «Kharkov Polytechnic Institute», Kharkіv
Institute of Animal Science UААS, Kharkіv
Determination of cooperation of the optimized parameters of cryopreservation of embryos of mouse during multifactor research is conducted. Optimum values of parameters have made: concentration of glycerin 10%; speed of cooling 0.3–0.6 0С/min; final temperature of cooling –30+35 0С; time of endurance before immersing in liquid nitrogen 0–5 minutes. By means of the dispersive analysis, influence force of researched parameters on safety depreservation object is determined: 0,18; 0,71; 0,45; 0,40, accordingly. For description of interrelation of researched parameters, on the basis of use of a vector method, surfaces of the response are constructed and factors of regress are determined: 26; 63; 10; 45 c.u., accordingly. On the basis of the researches the way of freezing of embryos is offered. Multifactorial optimization of parameters cryopreservation of mouse embryos has provided their safety at a level 88% at efficiency of realization of the given procedure 85%.
Key words: mouse embryos, cooling procedure, cryopreservation efficiency , influence force of parameters.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008