ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 19, No. 2, 2026
P. 33-41 , Bibliography 21, Engl.
UDC: 581:582.66: 602:547: 543.4
doi:https://doi.org/10.15407/biotech19.02.033
INVESTIGATION OF EXTRACTS OF Amaranthus retroflexus HERB, Amaranthus hypochondriacus HERB, AND CALLUS BIOMASS (cv. ‘Lera’) FOR TOTAL PHENOL AND FLAVONOID CONTENT
K. I. HUTSKO, R. O. PETRINA
Lviv Polytechnic National University, Ukraine
Amaranthus is a pseudocereal plant with a high content of proteins and fatty acids in its seeds, andpolyphenolic compounds and flavonoids in its leaves.
Aim. To investigate the influence of extractant type, hydromodule, extraction time, temperature,
and extraction method on the extraction of polyphenolic compounds and flavonoids from A. retroflexus herb, A. hypochondriacus herb, and callus biomass.
Methods. The content of polyphenolic compounds and flavonoids was determined using a spectrophotometric method.
Results. The optimal extraction conditions for A. retroflexus herb were a 40% and 80% aqueous ethanol solution, a hydromodule of 1:10, an extraction temperature of 20 ºC, and shaking at 100–200 rpm.
For A. hypochondriacus callus biomass, the optimal conditions were a 40% aqueous ethanol solution and a hydromodule of 1:10. For A. hypochondriacus herb, the optimal conditions were a 50% aqueous ethanol solution, a hydromodule of 1:10, an extraction temperature of 30–40 °C, and magnetic stirring at 400 and 800 rpm for the maximum yield of flavonoids and polyphenolic compounds, respectively. Extraction was carried out for up to 270 h by static maceration.
Conclusions. Optimal extraction parameters were established to ensure maximum yields of polyphenolic compounds and flavonoids from A. retroflexus herb, A. hypochondriacus herb, and callus.
Keywords: plant extract, callus biomass, polyphenolic compounds, flavonoids, maceration.
Romana Petrina https://orcid.org/0000-0002-5416-1999
Kateryna Hutsko https://orcid.org/0000-0003-3307-5650
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2026
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