ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta Т. 19, No. 2, 2026
P.  42-52, Bibliography 50, Engl.
UDC:  [582.284:577.151.54]: 664.292
doi: https://doi.org/10.15407/biotech19.02.054

Full text: (PDF, in English)

PECTINOLYTIC ACTIVITY OF THE MACROMYCETE TRAMETES HIRSUTA (WULFEN) LLOYD UNDER SURFACE AND SUBMERGED CULTIVATION

Zubyk P.R., Dzygun L.P., Klechak I.R.

National Technical University of Ukraine "Igor Sikorsky Kyiv Polytechnic Institute"

Pectinolytic enzymes are of considerable industrial importance because they degrade pectin in plant cell walls. Recently, increasing attention has been directed toward basidiomycetes, particularly the genus Trametes, which are capable of synthesizing complex enzymatic systems; however, their potential for pectinase production remains insufficiently explored.

This study aimed to investigate the growth and enzymatic activity of the basidiomycete Trametes hirsuta under surface and submerged cultivation in order to expand the biotechnological potential of these organisms as pectinase producers.

Methods: The study involved six strains of the macromycete Trametes hirsuta deposited at the Institute of Botany of the National Academy of Sciences of Ukraine. A peptone–yeast extract medium supplemented with pectin (solid nutrient medium) was used for surface cultivation. Mycelial growth was measured daily, and pectinase activity was assessed using a cetyltrimethylammonium bromide (CTAB)- based method. Submerged cultivation was performed in a glucose–peptone–yeast extract medium for 14 days, after which protein content, dry biomass, and pectinolytic enzyme activity were analyzed. Statistical analysis included Student’s t-test and Duncan’s multiple-range test using R and Excel.
Results. The T. hirsuta strains 5018 and 5137 demonstrated more active growth on pectin-containing media. Overall, the overgrowth period ranged from 5 to 9 days, depending on the strain. The highest radial growth rate was recorded for strain T. hirsuta 1569 (10.25 mm/day). The pectinase activity index (PAI) was determined, with strain T. hirsuta 1569 exhibiting the highest value (1.08 ± 0.04), indicating its strong potential for pectinolytic enzyme synthesis.

Conclusions. During submerged cultivation, strain T. hirsuta 1569 exhibited high polygalacturonase (1.28 ± 0.13 U/mL) and pectinesterase (0.31 ± 0.04 U/mL) activities. In contrast, strain T. hirsuta 338 exhibited significantly lower enzymatic activities and was therefore excluded from further experiments.

Keywords: Trametes, pectinolytic enzymes, growth rate, pectinase activity, polygalacturonase, pectinesterase, biotechnology

Zubyk P.R: https://orcid.org/0000-0003-0435-0254
Dzygun L.P.: https://orcid.org/0000-0002-4900-5887
Klechak I.R.: https://orcid.org/0000-0002-7382-0259

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2026