ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta Т. 16, No. 6 , 2023
P. 17-33, Bibliography 33, Engl.
UDC:: 579.663
DOI: https://doi.org/10.15407/biotech16.06.017

Full text: (PDF, in English)

INFLUENCE OF BIOLOGICAL INDUCTORS ON THE SYNTHESIS AND BIOLOGICAL ACTIVITY OF MICROBIAL METABOLITES

 T.P. Pirog^1,2, M.S. Ivanov¹

¹National University of Food Technologies, Kyiv, Ukraine
²Institute of Microbiology and Virology of NASU, Kyiv, Ukraine

The increasing antibiotic resistance is a severe concern for humanity. Co-cultivation of microorganisms is a promising method for obtaining new secondary antimicrobial metabolites. An effective strategy for the co-cultivation of microorganisms involves using certain biological inductors.

The review aimed to summarize existing scientific research in the literature related to the influence of physiologically different types of biological inductors on the synthesis and biological activity of microbial secondary metabolites.

An analysis of the literature showed that in such studies, either live or inactivated cells of the inductor were added to the culture medium at significantly lower concentrations compared to the producer cells of the final metabolites or the supernatant (filtrate) after cultivation of a competitive microorganism was used as an inductor.
According to the literature and our experimental studies, using inductors was a practical approach not only for intensifying the synthesis of bacteriocins, surfactants, and antibiotics but also for increasing their biological activity. Additionally, it often leads to the production of novel antimicrobial compounds that are not typical for the producer.

However, the mechanisms of the effect of inductors on the synthesis of biologically active secondary metabolites required further research, as the literature suggests that their introduction into the cultivation medium of the producer did not always lead to an intensification of the synthesis of the final product. Moreover, the biological activity of secondary metabolites depended on the cultivation conditions of the producer, including the presence of biological inductors in the culture medium. Therefore, it was essential to conduct further research on the interaction between producers and competitive microorganisms to regulate the biological activity of the synthesised metabolites. In addition, there was a necessity to search for more cost-effective substrates for the biosynthesis of secondary metabolites, optimize the composition of the culture medium, and expand the range of both pro- and eukaryotic inductors.

Key words: co-culture, inductor, physiological state of the inductor, antimicrobial metabolites.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2023