DYNAMICS OF THE ANTIOXIDANT ACTIVITY AND PHENOLIC CONSTITUENTS IN SUBMERGED CULTURES OF XYLARIA SPECIES Atamanchuk A.R.1, Bisko N.A.

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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

cover biotech acta general

Biotechnologia Acta Т. 16, No. 6 , 2023
P. 82-87, Bibliography 16, Engl.
UDC:: 582.282.195.2:579.222
DOI: https://doi.org/10.15407/biotech16.06.082

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DYNAMICS OF THE ANTIOXIDANT ACTIVITY AND PHENOLIC CONSTITUENTS
IN SUBMERGED CULTURES OF XYLARIA SPECIES

A. R. Atamanchuk, Bisko N.A..

Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Kyiv, Ukraine

Purpose. This study was conducted to enhance comprehension of the dynamic process synthesis of phenolic compounds by representatives of the genus Xylaria and the correlation between phenol content and antioxidant properties found in biomass and culture liquid during submerged cultivation.

Methods. Cultivation of Xylaria polymorpha and Xylaria longipes fungal strains from the IBK Mushroom Culture Collection was carried out on a glucose-yeast-peptone nutrient medium under submerged conditions. Harvesting of biomass and culture liquid was done on the 3rd, 5th, 7th, and 9th day of cultivation, followed by extraction with ethyl acetate. The total phenol content of extracts was determined using the Folin–Ciocalteu method. The antioxidant potential was evaluated through the DPPH assay.

Results. Findings revealed that the accumulation of phenolic compounds by fungal species of the Xylaria genus was specified on a strain level. Notably, X. longipes strains exhibited higher production of phenolic constituents compared to X. polymorpha and demonstrated superior antioxidant activity. Furthermore, a strong correlation was established between the dynamics of polyphenol accumulation and antioxidant activity in both mycelial biomass and culture liquid.

Conclusions. Natural phenolic compounds with antioxidant properties were extracted from the biomass and culture liquid of the studied strains. Significantly higher concentrations of phenolic compounds and values of antioxidant activity were found in the biomass compared to the culture liquid. The results indicated that a later day of cultivation was not necessarily equivalent to the production of more phenols, emphasizing the need for a comprehensive assessment of these compounds' accumulation and the dynamic study of the related parameters.

Key words: Xylaria, phenolic compounds, antioxidants, dynamics, biomass, culture liquid.

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