ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 11, No 4, 2018
https://doi.org/10.15407/biotech11.04.068
Р. 68-72, Bibliography 8, English
Universal Decimal Classification: 79.841.95:577.2.08:579.252.5:619:616.98-076
DEVELOPMENT OF RECOMBINANT POSITIVE CONTROL FOR Francisella tularensis DETECTION BY Q-PCR
O. B. Zlenko, A. P. Gerilovych
National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine” of the National Academy of Agrarian Sciences of Ukraine, Kharkiv
The aim of the work was to construct and test the recombinant positive control for F. tularensis detection by a real-time polymerase chain reaction (qPCR). The molecular TA-cloning of pTZ57_F/R plasmid ligated with tul4 gene PCR product into DH5α E. coli. The minimal detection level in qPCR was one copy number per reaction. The obtained positive control was highly sensitive, specific and safe qPCR in the laboratory tularemia diagnostics.
Key words: . ecombinant positive control, qPCR., tularemia, molecular cloning.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018
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