ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 7, no 5, 2014
https://doi.org/10.15407/biotech7.05.071
Р. 71-76, Bibliography 11, Russian.
Universal Decimal classification: 582.548.25: 57.085.23
THE PECULIARITIES OF CLONAL MICROPROPAGATION OF CANNA GARDEN (Canna Χ Hybrida hort.)
Tevfik A. Sh., Mitrofanova I. V., Kuzmina T. N.
Nikitsky Botanical Garden — National Scientific Centre of the National Academy of Agrarian Sciences of Ukraine, Crimea Autonomic Republic, Ukraine
Regenerants of Canna (Canna x hybrida hort.) cvs. Suevia, Dar Vostoka and Livadia have been obtained. Dependence of viability and regenerative capacity of Canna microshoots on genotype has been found. The optimal concentrations of growth regulators that influence on morphogenesis in vitro have been determined. At the micropropagation stage of different cultivars of Canna it needs the addition of 1.24–1.91 mg/l thidiazuron to culture medium. The active rooting of Canna microshoots cvs. Dar Vostoka and Suevia on the culture medium Murashige & Skoog and Pierik with auxins β-indol-3- acetic acid and α-naphthylacetic acid have been observed. The ability of Canna cv. Suevia microshoots to spontaneous root formation has been shown. The formation of meristemoids in culture in vitro of Canna explants have been confirmed by the results of histological investigations.
Key words: canna, regeneration, meristemoid.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014
References
1. Mitrofanova I. V. Microclonal propagation of subtropical and tropical fruits (overview). Trudy Nikitskogo botanitcheskogo sada. 1997, V. 119, P. 63–95. (In Russian).
2. Mitrofanova I. V. Somatic embryogenesis and organogenesis as a base of biotechnology of obtaining and conservation perennial horticultural plants. Kyiv: Ahrarna nauka. 2011, 344 p. (In Russian).
3. Dashkeev E. A. Canna in Moldova. Kishinev: Shtinitsa. 1975, 65 p. (In Russian).
4. Feofilova G. F. Assortment and growing technology of promising canna varieties for homeland’s south regions. Trudy Nikitskogo botanitcheskogo sada. 1991, V. 112, P. 41–50. (In Russian).
5. Butenko R. G. Culture of isolated tissues and physiology of plants morphogenesis. Moskva: Nauka. 1964, 272 p. (In Russian).
6. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962, 15(3), 473–497.
http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
7. Pierik R. L. M. Anthurium andraeanum plantlets produced from callus tissues cultivated in vitro. Physiol Plant. 1976, N 7, P. 80–82.
http://dx.doi.org/10.1111/j.1399-3054.1976.tb01876.x
8. Lakin G. F. Biometrics: a textbook for universities biological specialties. Moskva: Vysshaia shkola. 1990, 352 p. (In Russian).
9. Pausheva Z. P. Handbook of plants cytology. Moskva: Kolos. 1970, 255 p. (In Russian).
10. Zhinkina N. A., Voronova O. N. The coloring method of embryological preparations. Botanicheskii zh. 2000, 85(6), 168–171. (In Russian).
11. Tevfik A. Sh. Horticultural Canna (Canna x hybrida hort.) plants regeneration in culture of vegetative buds in vitro. Trudу Nikitskogo botanitcheskogo sada. 2012, V. 134, P. 426–435. (In Russian).