ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 7, no 5, 2014
https://doi.org/10.15407/biotech7.05.062
Р. 62-70, Bibliography 23, English.
Universal Decimal classification: 602.1:519.673+602.3:579.864
OPTIMIZATION OF TANNASE POSITIVE PROBIOTIC PRODUCTION BY SURFACE RESPONSE METHODOLOGY
Yumnam S.1, Prasanna B.1, Oriabinska L. B.2, Khrokalo L. A.2, Dugan O. M.2
1National Institute of Technology Karnataka, India
2National Technical University of Ukraine «Kyiv Polytechnic Institute», Kiyv, Ukraine
Study in conditions in vitro of eight Lactobacillus strains procured from culture repositories for their probiotic potential and extracellular tannase activity was the aim of the research. Based upon acid, bile salt tolerance and antibiotic resistance L. plantarum MTCC 2621 with high tannase activity was selected for production studies. Optimization of nutrient medium in 3 L bioreactor was optimized by Surface Response Methodology based on the Full Factorial Central Composite Design. A factorial design 23 augmented by 6 axial points (α= 1.68) and six replicates at the center point was implemented in 20 experiments. The optimized conditions were found to be pH 5.69, contain of lactose 128.58 g/l, peptone 8 g/l. A tenfold increase in the biomass production was observed using the optimized nutrient medium in bioreactor as compared to initial MRS medium.
Key words: Lactobacillus, optimization of medium, probiotic, response surface methodology.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014
References
1. FAO / WHO Food and Agricultural Organization of the United Nations and World Health Organization: Guidelines for the evaluation of probiotics in food. 2002, 11 р. Available at http: //www.fao.org/es/esn/food/foddanfood_probio.
2. Jacobsen C., Rosenfeldt Nielsen V., Hayford A., Moller P., Michaelsen K., Paerregaard A., Sandstr?m B., Tvede M., Jakobsen M. Screening of probiotic activities of forty seven strains of Lactobacillus spp. by in vitro techniques and evalution of the colonization ability of five selected strains in humans. Appl. Environ Microbiol. 1999, 659(11), 4949–4956.
3. Kaila M., Isolauri E., Soppi E., Virtanen E., Laine S., Arvilommi H. Enhancement of the circulating antibody secreting cell response in human diarrhea by a human Lactobacillus strain. Ped. Res. 1992, V. 32, P. 141–144.
http://dx.doi.org/10.1203/00006450-199208000-00002
4. Nagao F., Nakayama M., Muto T., Okumura K. Effects of a fermented milk drink containing Lactobacillus casei strain Shirota on the immune system in healthy human subjects. Biosci. Biotechnol. Biochem. 2000, 64(12), 2706–2708.
http://dx.doi.org/10.1271/bbb.64.2706
5. Black F., Einarsson K., Lidbeck A., Orrhage K., Nord C. Effect of lactic acid producing bacteria on the human intestinal microflora during ampicillin treatment. Scand. J. Infect. Disease. 1991, V. 2, P. 247–254.
http://dx.doi.org/10.3109/00365549109023408
6. Gismondo M. R., Drago L., Lombardi A. Review of probiotics available to modify gastrointestinal flora. Int. J. Antimicrob. Agents. 1999, V. 12, P. 287–292.
http://dx.doi.org/10.1016/S0924-8579(99)00050-3
7. Gupta P., Andrew H., Kirschner B. S., Guandalini S. Is Lactobacillus GG helpful in children with Crohn’s disease? Results of a preliminary, open-label stud. J. Ped. Gastroenterol. Nutr. 2000, V. 31, P. 453–457.
http://dx.doi.org/10.1097/00005176-200010000-00024
8. Guslandi M., Mezzi G., Sorghi M., Testoni P. A. Saccharomyces boulardii in maintenance treatment of Crohn’s disease. Digestive Dis. Sci. 2000, V. 45, P. 1462–1464.
http://dx.doi.org/10.1023/A:1005588911207
9. Hamilton-Miller J. A review of clinical trials of probiotics in the management of inflammatory bowel disease. Infect. Dis. Rev. 2001, V. 3, P. 83–87.
10. Mattila-Sandholm T. Blum S., Collins J., Crittenden W. de Vos R., Dunne C., Fondin R., Grenov G., Isolauri E. Probiotics: towards demonstrating efficacy. Trends Food Sci. Technol. 1999, V. 10, P. 393–399.
http://dx.doi.org/10.1016/S0924-2244(00)00029-7
11. Higdon R., Frei A. Tea catechins and polyphenols: health effects, metabolism and anti-oxidant functions. Crit. Rev. Food. Sci. Nutr. 2003, V. 43, P. 89–143.
http://dx.doi.org/10.1080/10408690390826464
12. Lambart A., Elias V. The anti-oxidant and pro-oxidant activities of green tea polyphenols: a role in cancer prevention. Arch. Biochem. Biophys. 2010, V. 501, P. 65–72.
http://dx.doi.org/10.1016/j.abb.2010.06.013
13. Lekha P. K., Lonsane B. K. Production and application of tannin acyl hydrolase: state of the art. Adv. Appl. Microbiol. 1997, V. 44, P. 215–260.
http://dx.doi.org/10.1016/S0065-2164(08)70463-5
14. Bhat T. K., Singh B., Sharma O. P. Microbial degradation of tannins-A current perspective. Biodegradation. 1998, V. 9, P. 343–357.
http://dx.doi.org/10.1023/A:1008397506963
15. Osawa R., Kuroiso K., Goto S., Shimizu A. Isolation of tannin-degradation Lactobacilli from humans and fermented foods. Appl. Environ. Microb. 2000, V. 66, P. 3093–3097.
http://dx.doi.org/10.1128/AEM.66.7.3093-3097.2000
16. Thirabunyanon M., Boonprasom P., Niamsup P. Probiotic potential of lactic acid bacteria isolated from fermented dairy milks on antiproliferation of colon cancer cells. Biotechnol Lett. 2009, V. 3, P. 571–576.
http://dx.doi.org/10.1007/s10529-008-9902-3
17. Pennacchia C., Ercolin D., Blaiotta G., Pepe O., Mauriello G., Villani F. Selection of Lactobacillus strains from fermented sausages for their potential use as probiotics. Meat Sci. 2004, V. 67, P. 309–317.
http://dx.doi.org/10.1016/j.meatsci.2003.11.003
18. Zoumpopoulou G., Foligne B., Christodoulou K., Grangette C., Pot B., Tsakalidou E. Lactobacillus fermentum ACA-DC 179 displays probiotic potential in vitro and protects against trinitrobenzene sulfonic acid (TNBS)-induced colitis and Salmonella infection in murine models. Int. J. Food Microbiol. 2008, V. 121, P. 18–26.
http://dx.doi.org/10.1016/j.ijfoodmicro.2007.10.013
19. Iyer R., Tomar S. K., Kapila S., Mani J., Singh R. Probiotic properties of follate producing Streptococcus thermophiles strain. Food Research Int. 2010, V. 43, P. 103–110.
http://dx.doi.org/10.1016/j.foodres.2009.09.011
20. Maragkoudakis P. A., Zoumpopoulou G., Miaris C., Kalantzopoulos G., Pot G., Tsakalidou E. Probiotic potential of Lactobacillus strains isolated from dairy products. Int. Dairy J. 2006, 16(2), 189–199.
http://dx.doi.org/10.1016/j.idairyj.2005.02.009
21. Ei-Naggar M. Comparative study of probiotic cultures to control the growth of E. coli O 157: H 7 and Salmonella typhimurium. Asian Netw. Sci. Inf. Biotechnol. 2004, 3(2), 173–180.
22. Arici M., Bilgin B., Sagdic O., Ozdemir C. Some characteristics of Lactobacillus isolates from infant faeces. Food Microbiol. 2004, V. 21, P. 19–24.
http://dx.doi.org/10.1016/S0740-0020(03)00044-3
23. Verdenelli M. C., Ghelfi F., Silvi S., Orpianesi C., Cecchini C., Cresci A. Probiotic properties of Lactobacillus rhamnosus and L. paracasei isolated from human faeces. Eur J. Nutr. 2009, 48(6), 355–363.
http://dx.doi.org/10.1007/s00394-009-0021-2