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ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)

 6 2013

"Biotechnologia Acta" v. 6, no 6, 2013
Р. 71-76, Bibliography 21, Russian.
Universal Decimal classification: 546.26.043


І. І. Grynyuk1, S. V. Prylutska1, N. S. Slobodyanik1, О. Yu. Chunikhin2,  О. P. Matyshevska1

1Taras Shevchenko National University of Kyiv, Ukraine
2Palladin Institute of Biochemistry of National Academy Sciences of Ukraine, Kyiv

The distribution of pristine C60-fullerene nanoparticles by volume and number in water solution without chemical dispersants with different salt and protein composition was evaluated using the correlation spectroscopy analysis. It was shown, that water colloid solution contains C60-fullerene particles with hydrodynamic diameter from 38 to 600 nm, and the main peak position is at 43 nm. In saline solution (0,9% NaCl) the particle size distribution range enlarges that may result from the C60 aggregation. Albumin introducing into the medium promotes dispersed state of C60 nanoparticles and prevents their spontaneous as well as NaCl-dependent agglomeration. No obvious aggregation was detected when C60-fullerene in concentration 10–5 М was added to RPMI medium containing 5% fetal bovine serum. The data show that stable homogenous water colloid solution of pristine C60 fullerene could be used for studding cell respon ses in cultural medium.

Key words: C60-fullerene, photon correlation spectroscopy, albumin, agglomeration.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2013


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