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ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)

 6 2013

"Biotechnologia Acta" v. 6, no 6, 2013
Р. 77-85, Bibliography 26, Ukrainian.
Universal Decimal classification: 582.542.11:57.086.83

CALLUS FORMATION AND REGENERATION OF Deschampsia antarctica Desv. (Poaceae) IN CULTURE in vitro

О. M. Zagrychuk1, A. I. Herts1, N. M. Drobyk1, V. A. Kunakh2

1Volodymyr Hnatiuk Ternopil National Pedagogical University, Ukraine
2Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv

Conditions for induction of callus formation from root and stem explants and long-term maintenance of Deschampsia antarctica Desv. Tissue culture have been specified. Ability to callus formation depended on mineral composition of nutrient medium, combination of growth regulator concentrations, place of donor-plant vegetation and type of explant. The optimal for callus tissue generation was Gamborg, Eveleigh — B5 nutrient medium, supplemented with 0.9–1.0 mg/l 2.4-dichlorophenyl acetic acid and 0.09–0.1 mg/l of cytokinine benzylaminopurine. Callus formation potency from the root explants considerably exceeded (1.5–2 times) that of from stem ones. The shoots were derived through spontaneous indirect organogenesis. Regeneration efficiency was found to be affected by nutrient medium composition and callus origin. Regenerated shoots were rooted and conditions for growth of regenerated plants in vitro were specified.

Key words: Deschampsia antarctica Desv., callus formation, callus culture, spontaneous indirect regeneration in vitro, regenerated plants.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2013


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