ISSN 1995-5537
"Biotechnology" journal V. 5, No.16, 2012
Р. 78-85, Bibliography 10, Ukrainian.
Universal Decimal classification: 582.28+581.19+576.345+615.15
L. V. Panchak, O. Yu. Klyuchivska, M. V. Tsivinska,
R. S. Stoika, R. B. Lesyk, V. O. Antonyuk
Danylo Halytsky Lviv National Medical University
Institute of Cell Biology of National Academy of Sciences of Ukraine, Lviv
Ivan Franko Lviv National University
Scientific and Research Criminal Expertise Centre
of Ministry of Internal Affairs of Ukraine in Lviv region
The method of fractionation of the methanol extract from basidiomes of Lactarius pergamenus mushroom by the organic solvents and chromatography on the silica gel column is described. Identification of substance in the obtained fractions was carried out by mass-spectrometry. Antiproliferative activity was defined in the culture of murine leukemia cells of L1210 line. It was found that the fraction containing a substance with yellow-green fluorescence under UV light possessed the highest antiproliferative activity. This substance was identified as 3,14,15-trimethylfuranolactaranе-8-ol. Concomitant higher fatty acids and their ethers also play a noticeable role in cytotoxic effect. The toxic effect of trimethylfuranolactaran of L. pergamenus towards the cultured murine leukemia cells of L1210 line was many times weaker as compared to such effect of the alantolactone of the Inula helenium rhizomes. However, trimethylfuranolactaran is more effective cytostatic as compared to alantolactone. Thus, the sesquiterpen substances of fruit bodies of Lactarius mushrooms be perspective antiproliferative agents.
Key words: Lactarius pergamenus, methanol extraction, mass-spectrometry, 3,14,15-trime-thylfuranolactarane-8-ol, cytotoxic effect, murine leukemia cells of L1210 line.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008