SPECIES-SPECIFIC DETECTION OF CAUSATIVE AGENT OF ANTHRAX O. Yu. Limanskaya, L. А. Murtazaeva, A. P. Limanskii

Details: Hits: 310

ISSN 1995-5537

1 2012

"Biotechnology" journal V. 5, No. 1, 2012
Р. 92-99, Bibliography 24, Ukrainian.
Universal Decimal classification: 577.2:573.6

SPECIES-SPECIFIC DETECTION OF CAUSATIVE AGENT OF ANTHRAX

O. Yu. Limanskaya, L. А. Murtazaeva, A. P. Limanskii

Mechnikov Institute of Microbiology and Immunology of National Academy
of Medical Sciences of Ukraine, Kharkiv
National Scientific Center «Institute of Experimental and Clinical Veterinary Medicine»
of National Academy of Agrarian Sciences of Ukraine, Kharkіv

Nucleotide sequences were analyzed and dendrograms were obtained for memberes of Bacillus cereus sensu lato group (B. anthracis, B. sereus and B. thuringiensis) based on rpoB, plcr, ssp genes of chromosomal DNA, which may be the molecular genetic markers for their typing. Testing the primer set, which target is the ssp gene fragment characterized by hexanucleotide insertion only for B. anthracis isolates, has allowed differentiating B. anthracis bacteria reliably of closely related B. sereus and B. thuringiensis species by conventional polymerase chain reaction. It was shown that phylogenetic tree can be applied as an indicator of the reliability and accuracy of the taxonomical classification of species and subspecies of pathogens. Unlike all the analyzed B. anthracis strains, that form a monophyletic cluster on a dendrogram, there are B. sereus strains that are phylogenetically more related to B. thuringiensis strains, as well as B. thuringiensis strains, that are more related phylogenetically to B. sereus strains, suggesting that they should be reclassified.

Key words: genotyping, anthrax causative agent, Bacillus anthracis, polymerase chain reaction.