ISSN 1995-5537
"Biotechnology" journal V. 5, No. 1, 2012
Р. 100-106, Bibliography 18, Russian.
Universal Decimal classification: 578.001.53:543.45
IMMUNOSENSORY TEST-SYSTEM FOR DETECTION OF ANTIBODIES
TO THE EPSTEIN-BARR VIRUS
N. V. Nesterova, S. L. Rybalko, S. D. Zagorodnya, G. V. Baranova, A. V. Golovan
Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv
Gromashevsky Institute of Epidemiology and Infectious Diseases of National Academy
of Medical Sciences of Ukraine, Kyiv
Contemporary level of molecular biology development in combination with the achievements of physics and chemistry have defined a new trend in diagnostics of diseases which used immunosensory test-systems elaborated on the chemical and optical basis. In particular, attention was drawn to biosensors based on surface plasmon resonance effect, which belong to a class of optical ones.
Data on the express identification of antibodies to Epstein-Barr virus in sera of patients obtained with an optoelectronic device, «Plasmon 6», are given in the article. The series of experimental immunosensors for express diagnostics developed and basic indices of their diagnostic quality were worked through. The negative and positive panels of sera were created, which were used to evaluate the diagnostic specificity and sensitivity of the elaborated immunosensory test-system. Diagnostic specificity of the elaborated test-system was 100%, diagnostic sensitivity — 95%, the reproducibility of the results — 99%. The conditions and possible storage life of the immunosensors were investigated. The period of their storage was 6 months that indicated on stability of the elaborated immunosensors. The experimental testing of the samples of the worked out immunosensory test system shown their sufficiently effectiveness and specificity for detection of antibodies to Epstein-Barr virus and it could be used to diagnostics of the diseases caused by the virus.
Key words: Epstein-Barr virus, surface plasmon resonance, immunosensory test-system.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008