ISSN 1995-5537

"Biotechnology" journal V. 5, No.16, 2012
Р. 70-77, Bibliography 10, Ukrainian.
Universal Decimal classification: 612.398:547.965

AUXOTROPHITY OF PRODUCENTS OF LYSIN

G. S. Andriyash, G. M. Zabolotna, S. M. Shulga

State organization «Institute of Food  Biotechnology and Genomics»
of National Academy of Sciences of Ukraine, Kyiv

The investigation of lysine producer strains of «Collections of microbial strains and plant lines for food and agricultural biotechnology» of the State organization «Institute of Food Biotechnology and Genomics» of National Academy of Sciences of Ukraine was carried out. Clonal analysis of the strains was done after batch fermentation of producers on molasses medium. It was found that Brevibacterium sp. 90, Brevibacterium sp. 90H, and Brevibacterium sp. XI strains gave colonies scission into two types. All the clones were estimated by biosynthetic activity relatively lysine. Mutational changes were observed in the clones some of which remained dependent on leucine and get a new auxotrophity for threonine, methionine, tryptophan, isoleucine and homoserine. It was shown that only some clones were resistant to S-aminoethyl-L-cysteine — analogue of lysine. Clones on biosynthetic activity relative to the target amino acid were selected for further research with the aim to improve lysine biosynthesis by Brevibacterium producer strains.

Key words: amino acids, auxotrophity, biosynthesis, clones, lysine.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008