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ISSN 1995-5537

1 2012

"Biotechnology" journal V. 5, No. 1, 2012
Р. 53-61, Bibliography 14, Ukrainian.
Universal Decimal classification: 557.152.1+53


N. S. Rogaleva, L. V. Shkotova, O. V. L’vova, V. V. Garbuz, V. B. Muratov, Т. І. Duda, O. O. Vasil’ev, Ya. І. Korpan, О. А. Biloivan

Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv
Taras Shevchenko National University of Kyiv
Frantsevich Institute for Problems of Materials Science, Kyiv
National Technical University of Ukraine «Kyiv Polytechnic Institute»

To improve analytical characteristics of amperometric biosensors for glucose determination, aminated and carboxylated multiwalled carbon nanotubes were applied. Attested samples of nanotubes were suspended and used for modification of amperometric transducers С220АТ of DropSens production and bio-selective membranes. The glucose oxidase membranes were formed on the surface of working electrode by several methods: covalent crosslinking and coimmobilization with horseradish peroxidase in bovine serum albumin gel in glutaraldehyde vapors; electropolymerization of poly(3,4-ethylenedioxythiophene) in the conductive polymer film. Electrochemical measurements were performed using the device ?Stat 200 of DropSens production. The developed amperometric biosensors with a membrane with carbon nanotubes have been shown to be advantageous as compared to those without carbon nanotubes in terms of higher sensitivity, lower working potential, and wider dynamic range of the target analyte detection. The optimized biosensor was used for detection of glucose in real wine samples. A reliable correlation was observed between the results of biosensor method proposed and those obtained by high performance liquid chromatography.

Key words: multiwalled carbon nanotube, amperometric biosensor, glucose, glucose oxidase, nanocomposite membranes.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008