ISSN 1995-5537
"Biotechnology" journal V. 4, No. 6, 2011
Р. 51-59, Bibliography 33, Russian.
Universal Decimal classification: 621.014.46(546.57+546.56:66-911.38).001.5
STRUCTURE, PROPERTIES AND TOXICITY OF NANOPARTICLES
OF SILVER AND COPPER OXIDES
Institute of Occupational Health of National Academy of Medical Sciences of Ukrainу, Kyiv;
Paton Institute of Electro-welding of National Academy of Sciences of Ukraine, Kyiv;
Chuyko Institute of Surface Chemistry of National Academy of Sciences of Ukraine, Kyiv;
Palladin Institute of Biochemisry of National Academy of Sciences of Ukraine, Kyiv
Currently, a number of general methods for nanoparticles synthesis have been created. In terms of opportunities for industrial production of nanomaterials in Ukraine today, the most advanced is nanotechnology based on physical methods of their obtaining. Therefore today finding stable organic colloidal dispersions stabilized by surfactants and standardization of the experiments techniques in vitro and in vivo, development of safety criteria, and acceptable thresholds for toxicity of metal nanoparticles is an urgent problem. Physicochemical properties of nanoparticles of silver and copper oxides in colloidal solutions of albumin, polyvinylpyrrolidone, and dextran have been studied. Cytotoxicity of nanoparticles of silver and copper oxides in polyvinylpyrrolidone and dextran was evaluated. Toxicity of silver oxide nanoparticles was assessed during per os subacute experiment on Wistar rats. High toxicity of 46.6 mg/l concentration of silver oxide nanoparticles was detected. Biochemical study of enzyme system activity, measurement of trace elements content in whole blood and morphological study of blood cellular composition enabled to reveal minor changes in the composition of blood cells and amount of trace elements, as well as a significant reduction in ceruloplasmin activity and increased activity of ATP and zincprotoporphirine in response to per os subacute exposure of silver oxide nanoparticles with a size of 32 nm and 2.5 mg/l concentration.
Key words: nanoparticles of silver and copper oxides, albumin, polyvinylpyrrolidone, dextran, microelements, enzymes.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008