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ISSN 1995-5537

2011no6

"Biotechnology" journal V. 4, No. 6, 2011
Р. 60-67, Bibliography 41, Ukrainian.
Universal Decimal classification: 579.8:582.284

GROWTH OF Ganoderma applanatum (Pers.) Pat. AND G. lucidum (Curtis) P. Karst. STRAINS AND SYNTHESIS OF POLYSACCHARIDES IN SUBMERGED CULTURE

T. A. Krupodorova

Institute of Food Biotechnology and Genomics of National Academy of Sciences of Ukraine, Kyiv
Kholodny Institute of Botany of National Academy of Sciences of Ukraine, Kyiv

The production of biomass and exopolysaccharides for several strains of cultures Ganoderma applanatum (Pers.) Pat. and G. lucidum (Curtis) P. Karst. in submerged conditions on waste product of food industry of Ukraine — milk serum and starch meal are described. It was studied micromorphological characteristics of vegetative mycelium, that makes it possible to ensure the control of growth cleanness of cultures. Nutrient medium with milk serum for growth and synthesis of polysaccharides for investigated cultures was optimal. Maximal content of biomass (17,2±0,1 g/l) was produced G. applanatum 1572 on the 11th day of cultivation, G. lucidum 1621 — 29,6 ± 0,4 g/l on then 5th day grown. On the 11th day of growth the highest amounts of exopolysaccharides in G. lucidum 1621 — 10,0 ± 0,1 g/l and in G. applanatum 1572 — 9,1 ± 0,1 g/l were found.

Key words: morphology, G. applanatum, G. lucidum, biomass, exopolysaccharides, submerged conditions.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008