NUTRIENT MEDIA FOR in vitro CULTIVATION OF Ungernia victoris Vved. ex Artjushenko TISSUES O. M. Bublyk, I. O. Andreev, К. V. Spiridonova, L. P. Mozhylevska, V. A. Kunakh

Details: Hits: 357

ISSN 1995-5537

2011no6

"Biotechnology" journal V. 4, No. 6, 2011
Р. 68-73, Bibliography 15, Ukrainian.
Universal Decimal classification: 581.143.6: 582.573.21+575.22

NUTRIENT MEDIA FOR in vitro CULTIVATION OF Ungernia victoris Vved. ex Artjushenko TISSUES

O. M. Bublyk, I. O. Andreev, К. V. Spiridonova, L. P. Mozhylevska, V. A. Kunakh

Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv

Two nutrient media proposed earlier to cultivate in vitro rare medicinal herb Ungernia victoris by the indices of direct regeneration and callusogenesis efficiencies, as well as in terms of genetic variability of the regenerants, organogenic and nonorganogenic culture were assessed under long-term culturing. Medium 5C1 was found to be suitable preferentially for callusogenesis, while medium 5C01 could be used for both callusogenesis and plant regeneration as well as for their long-term cultivation. Both media ensure relatively low level of genetic variability. The obtained results give the possibility to suggest the high efficiency of the developed methods for establishment of tissue culture and micropropagation of Ungernia victoris Vved. еx Artjushenko.

Key words: Ungernia victoris, micropropagtion, tissue culture, somaclonal variation.