ISSN 1995-5537
"Biotechnology" journal V. 4, No. 6, 2011
Р. 42-50, Bibliography 33, Russian.
Universal Decimal classification: 579.22:581.557
SOYBEAN LECTIN EFFECT ON METABOLISM AND SYMBIOTIC PROPERTIES OF Bradyrhizobium japonicum STRAINS
D. M. Sytnikov, О. D. Krugova, N. M. Mandrovskaya
Kholodnyi Institute of Botany of National Academy of Sciences of Ukraine, Kyiv;
Institute of Plant Physiology and Genetics of National Academy of Sciences of Ukraine, Kyiv
The effects of soybean seed lectin (SBA) on growth, metabolism and symbiotic properties expression of soybean nodule bacteria (Bradyrhizobium japonicum) strains with different activity (634b, 646, 21110 and 604k) were studied in laboratory and vegetative experiments. It was shown that preliminary incubation of rhizobia with host-plant lectin stimulated their growth and increased the peroxidase activity of strains independently of the potential symbiotic activity. Soybean lectin initiated the appearance of new proteins in bacteria cells of rhizobial active strain 646. It was established that specific plant lectin was able to stimulate or inhibit the nodulation and nitrogen-fixing of strains with different activity under «soybean - nodule bacteria» symbiosis condition. Thus, homologous lectin through metabolism and character of symbiotic properties of rhizobia is multidirectional in its action on the genetically determinated microsymbiont potential.
Key words: Bradyrhizobium japonicum, soybean lectin, soybean-rhizobial symbiosis, nitrogenase activity, antioxidant enzymes.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008