ISSN 1995-5537
"Biotechnology" journal Vol. 2, No. 2, 2009
Р. 111-117, Bibliography 16, Ukrainian.
Universal Decimal classification: 615.373: 535..394:620.266.1
Р. 111-117, Bibliography 16, Ukrainian.
Universal Decimal classification: 615.373: 535..394:620.266.1
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
National Agricultural University, Kyiv
Institute of Molecular Biology and Genetics,National Academy of Sciences of Ukraine, Kyiv
A possibility of application of optimal immune biosensor based on surface plasmon resonance for express determination of trichotecen mycotoxin T-2 in solution has been investigated. Two variants of T-2 toxin determination by surface plasmon resonance immunosensor method have been proposed. It was shown opportunity of «direct» determination of T-2 with the detection limit of about 20 ng/ml and for «competitive» mode with the detection limit on the level of about 1 ng/ml. The effectiveness of application of such organic compounds as polyelectrolytes and thiols for sensible surface modification of SPR spectrometer for T-2 analysis in solutions was studied. Direct determination by using dodecanethiol and polyelectrolyte for surface modification allows to determine T-2 toxin at levels of 5 ng/ml and 2 ng/ml accordingly in case of using both poly- and monoclonal antibodies.
Key words: immobilization, T 2 toxin, surface plasmon resonance, polyelectrolyte, dodecantiol.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008