1_2015(en)

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ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)

1 2015

"Biotechnologia Acta" v. 8, no 1, 2015;
https://doi.org/10.15407/biotech8.01.082
Р. 82-87, Bibliography 43, English
Universal Decimal classification: 579.222: 547.979.8

BIOTECHNOLOGICAL CONDITIONS OF VALVE PROSTHESES CREATING BY TISSUE ENGINEERING METHOD

A. G. Popandopulo¹, M. V. Savchuk¹, D. L. Yudickiy²

¹SI « Husak Institute of Urgent and Recovery Surgery», Donetsk, Ukraine
²Gorky Donetsk National Medical University, Donetsk, Ukraine

Nowadays, definitive treatment for the end-stage organ failure is transplantation. Tissue engineering is an up to date solution to create the effective substitute of the defective organ. It involves the reconstitution of viable tissue with the use of autologous cells grown on connective tissue matrix, which has been acellularized before. Basis for the prothesis should be morphologically and physically nonmodified, so in case of making vessel-valvular biological prosthesises the decellularized extracellular matrix is the best variant. The xenogeneic extracellular matrix is economically and ethically more useful. The possibility of preservation of the morphological and chemical properties of matrix structure initiates the process of programmed cell death. In contrast to necrosis, which is a form of traumatic cell death that results from acute cellular injury, apoptosis doesn’t cause the tissue damages. One of the ways of realizing the apoptosis is the usage of EDTA — chelate, which binds the Ca²⁺ ions.

Key words: tissue engineering, extracellular matrix, decellularization, apoptosis, chelate.

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ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)

1 2015

Biotechnologia Acta, V. 8, No 1, 2015
https://doi.org/10.15407/biotech8.01.071
P. 71-75, Bibliography 15, Ukrainian
Universal Decimal classification: 637.146.34:638.167

THE BIOMASS OF Streptococcus thermophilus
AND Bifidobacterium longum IN DAIRY MEDIUM WITH BEE POLLEN

N. N. Lomova ¹, O. O. Snezhko ², S. A. Narizhnyy¹

¹ Bila Tserkva National Agrarian University, Ukrainian
² Nacionalnyj universitet bioresursov i prirodopolzovanija Ukrainy, Kyiv

The present study was carried out to investigate the effect of adding different concentrations of bee pollen on the biomass of Streprococus thermophilus and B. longum in the dairy environment. Sampling, preparation and conducting of tests were performed by standard methods of analysis. The counts of Str. thermophilus and B. longum were carried out by using M17 and MRS agar media. The phases growth were determined graphically.

Established, that bee pollen stimulates the accumulation of biomass Str. thermophilus on 9–15%, and B. longum – on 2,3–12,7% in an amount of up to 0.2–1.0%. Bee pollen reduces the duration of the lag phase for both types of microorganisms almost to its complete disappearance (1.0%). Pollen (1%) prolong stationary phase for streptococci and bifidobacteria to 30% and 20%, respectively. And also, will provide the biomass in the amount of 6 ± 0,1×109 CFU/cm3 (Str. Thermophilus) and 2,8 ± 0,1×108 CFU/cm3 (B. longum).

Str. thermophilus and B. longum readily assimilate essential micronutrients pollen. Components of bee pollen can act growth stimulants (bifidogenic factor) for the studied strains. The data obtained will form the basis of biotechnology dairy drink with bee products.

Key words: biomass, bee pollen, streptococci, bifidobacteria.

References

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13. Lomova N. M., Narizhnyy S., Snizhko O. Influence of incorporating honey, royal jelly and pollen on the biotechnological processes of dairy drink. Vostochno-evropeiskii zhurnal peredovykh tehnologii. 2014, 2/12 (68), 62–65. (In Ukrainian).

14. Lomova N., Narizhnyy S., Snezhko O. Yoghurt enrichment with natural bee farming products. Ukrainian Food J. 2014, 3 (3), 405–411.

15. Pirt S. J., Walach M. Biomass yields of Chlorella from iron (Yx/Fe) in iron-limited batch cultures. Arch. Microbiol. 1978, 116 (3), 293–296.
http://dx.doi.org/10.1007/BF00417854

Details: Hits: 516

ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)

1 2015

Biotechnologia Acta, V. 8, No 1, 2015;
https://doi.org/10.15407/biotech8.01.063
P. 63-79, Bibliography 25, English
Universal Decimal classification: 544.431.122: 582.284.3: 628.93

INDUCTION OF ANTIMICROBIAL ACTIVITY OF SOME MACROMYCETES BY LOW-INTENSITY LIGHT

N. L. Poyedinok¹, O. B. Mykhaylova ², A. M. Negriyko ³, I. A. Dudka ², B. F. Vasilyeva ⁴, O. V. Efremenkova⁴

¹ Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
² Kholodny Botany Institute of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
³ Institute of Physics of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
⁴Gause Institute of New Antibiotics, Moscow, Russian Federation

The aim of the work was to study the induction of antimicrobial activity of macromycetes by low-intensity light of different wavelengths and coherence. The objects of investigation were the strains of Flammulina velutipes 3923, Pleurotus ostreatus 531, Ganoderma lucidum 1908 and G. applanatum 1552 from Mushrooms Collection of the Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, the test-cultures from Cultural Collections of the Gause Institute of New Antibiotics, All-Union Research Institute of Antibiotics and the All-Russian Collection of Industrial microorganisms. Helium-neon laser with a wavelength of 632.8 nm and an argon ion laser with wavelengths of 488.0 nm and 514.5 nm were used as a source of coherent visible light lasers. For obtaining incoherent light LEDs with emission at a wavelength of 490.0, 520.0 and 634.0 nm were used. It was found that short-term exposure of sowing mycelium by low intensity light with the energy density of 230 MJ/cm2 in the red and blue wavelength ranges reduced the cultivation period before the appearance of antimicrobial activity and induced the increasing of the culture fluid inhibitory activity against different test-cultures from 20 to 238%. Selected modes of antimicrobial activity photostimulation could be used in biotechnology of submerged cultivation of macromycetes for intensification of technological stages and increasing the yield of the final product.

Key words: macromycetes, low intensity lights, lasers, antimicrobial activity, light emitting diodes.

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ISSN 2410-7751 (Print)

1 2015

Biotechnologia Acta, V. 8, No 1, 2015;
https://doi.org/10.15407/biotech8.01.056
P. 56-62, Bibliography 19, English
Universal Decimal classification: 58.085.2:582.542.11:631.811.98

EFFECT OF SYNTHETIC AUXIN LIKE GROWTH REGULATORS ON CALLUS REGENERATIVE ABILITY OF COMMON WHEAT VC. ZYMOYARKA

I. R. Gorbatyuk ¹, A. V. Bavol ^{1, 2}, A. V. Holubenko ^{1, 3}, B. V. Morgun ^{1, 2}

¹ Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kyiv
² Institute of Plant Physiology and Genetics, National Academy of Science of Ukraine, Kyiv
³ Taras Shevchenko National University of Kyiv, Ukraine

The aim of the study was to determine the dependence of morphogenetic reactions of wheat callus tissues to content of syntetic growth regulators of auxin nature (picloram, dicamba) in the nutrient medium.

Apical meristems of Triticum aestivum wheat were the primary explants for callusogenesis. Basic culture medium MS supplemented by vitamins of Gamborg, dicamba at different concentrations (0.2, 0.4, 0.6 mg/l), and picloram (0.16; 0.25; 0.5 mg/l) was used for regeneration. It was established that dicamba at a concentration of 0.2 mg/l is the most effective for production of regenerants. It was also observed that at the concentration of 0.16 mg/l picloram there are the formation of the greatest number of morphogenic zones (60%) and a significant amount of plant-regenerants. Increased concentrations of picloram to 0.25 mg/l and 0.5 mg/l caused a decrease in the number of morphogenic islands: in the first case, 10%, and the second – 36.4%. Among the described options the MS medium supplemented with 0.5 mg/l 6-benzylaminopurine and 0.16 mg/l picloram was the most effective. Shoots obtained from callus culture were capable to form roots in vitro and adapt to septic conditions. Regenerated plants when cultivated in greenhouse showed high viability (over 75%) and reached the generative phase.
Key words: growth regulators, picloram, dicamba, Triticum aestivum, in vitro culture.

References

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ISSN 2410-7751 (Print)

Biotechnologia Acta, V. 8, No 1, 2015;
https://doi.org/10.15407/biotech8.01.049
P. 49-55, Bibliography 27, English
Universal Decimal classification: 577.218:577.29

TRANSFORMATION EFFECTIVENESS FOR ARABIDOPSIS THALIANA PLANTS BY DNA-CONSTRUCTIONS WITH SITE-SPECIFIC RECOMBINASE SYSTEM Cre/loxP

A. S. Sekan, S. V. Isaenkov

SO «Institute of food Biotechnology and Genomics of the National Academy of Sciences of Ukraine», Kyiv

Using of new approach with site-specific recombinase system Cre/loxP under the control of 35S-promoter to generate marker-free genetically modified plants was developed. The analysis of recombinase system was carried out during the next generation of Arabidopsis thaliana plants, produced by agrobacterium transformation method. For this purpose two types of DNA-constructions were used for establishing better variant. The histochemical analysis of the plants progeny T₁ transformed by both construct types was described. As a result of our work, it was established that the amount of marker-free transformants was arising during every next transformation offspring independently of the used construct type. The new strategy provides a simple and rapid way to eliminate swelective and marker genes.

Key words: site-specific recombinase system Cre/loxP, marker genes excision.

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17. Wang Y., Chen B., Hu Y., Li J., Lin Z. Inducible excision of selectable marker gene from transgenic plants by the Cre/lox site-specific recombination system. Transg. Res. 2005, 14 (5), 605–614.
http://dx.doi.org/10.1007/s11248-005-0884-9

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