4_2008(en)

Details: Hits: 84

ISSN 1995-5537

"Biotechnology" journal Vol. 1, No. 4, 2008
Р. 9-17, Bibliography 48, Ukrainian.
Universal Decimal classification 577.15

PROTEIN C: MECHANISMS OF FUNCTIONING AND PRODUCTION METHODS

D. D. Zhernosekov, T. V. Kurkina

Palladin Institutе of Biochemistry of National Academy of Sciences of Ukraine, Kyiv
Taras Schevchenko Kyiv National University

Protein C pathway is an important link in numerous physiological and pathophysiological processes of organism. The particular features of protein C structure and mechanisms of its activation are considered. Special attention is given to functioning of activated protein C, to its anticoagulant, antiinflammatory and profibrinolytical properties. Possible therapeutic application of protein C for the treatment of large number of pathologies characterized by protein C deficiency is observed. Current sources and approaches for protein C preparation are described.

Key words: protein C, hemostasis, anticoagulant, inflammation.

Details: Hits: 108

ISSN 1995-5537

"Biotechnology" journal Vol. 1, No. 4, 2008
Р. 76-84, Bibliography 20, Ukrainian.
Universal Decimal classification 577.15.543.555

ENZYME CONDUCTOMETRIC BIOSENSORFOR LACTOSE CONTENT DETERMINATION

V. M. Pyeshkova, O. Y. Saiapina, O. O. Soldatkin, O. L. Kukla, S. V. Dzyadevych

Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv
National Taras Shevchenko University of Kyiv
National Food Industry University, Kyiv
Institute of Semiconductor Physics of the National Academy of Sciences of Ukraine, Kyiv

Enzyme conductometric biosensor for lactose determination has been developed. Three-enzyme membrane (glucose oxidase, mutarotase, β-galactosidase) immobilized on the surface of conductometric transducer was used as biosensitive element. The analytical characteristic of the biosensor obtained were investigated. The time of lactose analysis in solution was 1–2 minutes, linear range was from 0,01 mM to 0,75 mM for glucose determination and from 0,01 mM to 1,25 mM for lactose determination. Dependence of responses of biosensor to substrate on pH, ionic strength, buffer capacity of work solution were studied, and the data of selectivity and storage stability were presented.

The developed conductometric biosensor is featured in high operational stability and signal reproducibility.

Key words: conductometric enzyme biosensor, lactose, glucose, glucose oxidase, mutarotase, β-galactosidase.

Details: Hits: 75

ISSN 1995-5537

"Biotechnology" journal Vol. 1, No. 4, 2008
Р. 69-75, Bibliography 74, Ukrainian.
Universal Decimal classification 541.13:577.112.087

BIOLUMINESCENT BACTERIA AS SENSOR ELEMENTS FOR THE HEAVY METALS IONS’ CONTENT DETECTION

A. M. Zadorozhnyaya, T. G. Gruzina, S. M. Dibkova, Z. R. Ulber

Ovcharenko Institute of Biocolloidal Chemistry of the National Academy of Sciences of Ukraine, Kyiv

It has been shown the doze-dependent inhibition influence of gold ions on Photobacterium phosphoreum В7071 (lux⁺) natural luminescence. These bacterial cells can serve as a sensor element for ionic gold content detection, for example, in wastewater of gold-mining and gold-processing manufactures for requirements compliance control. The Pseudomonas fragi T2(5) transconjugates, which contain hybrid plasmid (Zn^Rlux⁺) and which are capable of the high-specific of light emission as a result of the contact with zinc ions, have been derived by conjugate crossing method. It enables to use these transconjugates as sensor elements in the high-specific bioluminescent detection of qualitative and quantitative content of this metal in environmental objects.

Key words: bacteria, natural bioluminescence, induced bioluminescence, detection, gold, zinc, conjugate crossing.

Details: Hits: 82

ISSN 1995-5537

"Biotechnology" journal Vol. 1, No. 4, 2008
Р. 64-68, Bibliography 19, Russian.
Universal Decimal classification 664.231: 577 15

MODIFICATION OF WHEAT STARCH BY DIFFERENT AMYLASES

L. V. Kaprelyants, T. V. Shpirko, O. F. Pomazanova

Odessa National Academy of Food Technologies

Effect of different preparations of α-amylase on the wheat starch and its fractions has been studied. Oligosaccharides with different molecular mass have been detected in the samples. It has been shown that hydrolysates with low glucose equivalent have the thermally reversible properties under the determined interrelation between low- and high-polymeric fractions of the starch.

Key words: starch, amylases, hydrolysate, enzymatic hydrolysis, oligosaccharides.

Details: Hits: 75

ISSN 1995-5537

"Biotechnology" journal Vol. 1, No. 4, 2008
Р. 57-63, Bibliography 13, Ukrainian.
Universal Decimal classification 615.222:581.143.6

MICROCLONAL PROPAGATION OF UNGERNIA VICTORIS VVED. EX ARTJUSCHENKO

V. А. Kunakh, L. P. Mozhylevska, O. M. Bublyk, I. V. Kolonina, V. I. Muzyka

Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv
LTD «Ungernia», Moscow, Russia

Approaches to microclonal propagation of the rare medicinal plant Ungernia victoris both through the direct regeneration from the scale of bulb and via induction of regeneration from the long_term grown in vitro callus tissues have been developed for the first time ever. Conditions for multiplication and maintenance of in vitro generated microbulb-regenerants were specified as well.

Key words: Ungernia victoris, microclonal propagation, direct plant regeneration, induction of plant regeneration.

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