ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)
Biotechnologia Acta, V. 8, No 1, 2015
P. 71-75, Bibliography 15, Ukrainian
Universal Decimal classification: 637.146.34:638.167
THE BIOMASS OF Streptococcus thermophilus
AND Bifidobacterium longum IN DAIRY MEDIUM WITH BEE POLLEN
N. N. Lomova 1, O. O. Snezhko 2, S. A. Narizhnyy 1
1 Bila Tserkva National Agrarian University, Ukrainian
2 Nacionalnyj universitet bioresursov i prirodopolzovanija Ukrainy, Kyiv
The present study was carried out to investigate the effect of adding different concentrations of bee pollen on the biomass of Streprococus thermophilus and B. longum in the dairy environment. Sampling, preparation and conducting of tests were performed by standard methods of analysis. The counts of Str. thermophilus and B. longum were carried out by using M17 and MRS agar media. The phases growth were determined graphically.
Established, that bee pollen stimulates the accumulation of biomass Str. thermophilus on 9–15%, and B. longum – on 2,3–12,7% in an amount of up to 0.2–1.0%. Bee pollen reduces the duration of the lag phase for both types of microorganisms almost to its complete disappearance (1.0%). Pollen (1%) prolong stationary phase for streptococci and bifidobacteria to 30% and 20%, respectively. And also, will provide the biomass in the amount of 6 ± 0,1×109 CFU/cm3 (Str. Thermophilus) and 2,8 ± 0,1×108 CFU/cm3 (B. longum).
Str. thermophilus and B. longum readily assimilate essential micronutrients pollen. Components of bee pollen can act growth stimulants (bifidogenic factor) for the studied strains. The data obtained will form the basis of biotechnology dairy drink with bee products.
Key words: biomass, bee pollen, streptococci, bifidobacteria.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008
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