ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)

Biotechnologia Acta, V. 8, No 1, 2015; 
https://doi.org/10.15407/biotech8.01.063
P. 63-79, Bibliography 25, English
Universal Decimal classification: 544.431.122: 582.284.3: 628.93

INDUCTION OF ANTIMICROBIAL ACTIVITY OF SOME MACROMYCETES BY LOW-INTENSITY LIGHT

N. L. Poyedinok ¹, O. B. Mykhaylova ², A. M. Negriyko ³, I. A. Dudka ²,  B. F. Vasilyeva ⁴, O. V. Efremenkova ⁴

¹ Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
² Kholodny Botany Institute of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
³ Institute of Physics of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
⁴ Gause Institute of New Antibiotics, Moscow, Russian Federation

The aim of the work was to study the induction of antimicrobial activity of macromycetes by low-intensity light of different wavelengths and coherence. The objects of investigation were the strains of Flammulina velutipes 3923, Pleurotus ostreatus 531, Ganoderma lucidum 1908 and G. applanatum 1552 from Mushrooms Collection of the Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, the test-cultures from Cultural Collections of the Gause Institute of New Antibiotics, All-Union Research Institute of Antibiotics and the All-Russian Collection of Industrial microorganisms. Helium-neon laser with a wavelength of 632.8 nm and an argon ion laser with wavelengths of 488.0 nm and 514.5 nm were used as a source of coherent visible light lasers. For obtaining incoherent light LEDs with emission at a wavelength of 490.0, 520.0 and 634.0 nm were used. It was found that short-term exposure of sowing mycelium by low intensity light with the energy density of 230 MJ/cm2 in the red and blue wavelength ranges reduced the cultivation period before the appearance of antimicrobial activity and induced the increasing of the culture fluid inhibitory activity against different test-cultures from 20 to 238%.  Selected modes of antimicrobial activity photostimulation could be used in biotechnology of submerged cultivation of macromycetes for intensification of technological stages and increasing the yield of the final product.

Key words: macromycetes, low intensity lights, lasers, antimicrobial activity, light emitting diodes.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2015