ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 17, No. 2 , 2024
P. 36-37, Bibliography 9, Engl.
UDC::57.08+543.4
DOI: https://doi.org/10.15407/biotech17.02.036
ABSORPTION AND LUMINESCENCE PROPERTIES OF ACID AND SALT FORMS OF MONONUCLEOTIDES, THEIR COMPONENTS AND COMPLEXES WITH D-MANNITOL AT ROOM TEMPERATURE
M.A. Dotsenko1, R.O. Nikolaiev1, Z.YU. Tkachuk1
1Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv
Aim. The aim of this work was to analyze and compare spectral properties of aqueous nucleotide solutions in conditions close to biological systems. We studied the absorption and luminescence (Ex and Em fluorescence and Em phosphorescence) of monoribonucleotides, their disodium salts, bases and nucleosides, and mixes with D-mannitol dissolved in water at room temperature.
Methods. There were measured absorbance spectra using a Specord 210plus instrument and fluorescence excitation and emission and phosphorescence spectra using Horiba Fluoro Max 4+ instruments.
Results. There were obtained the absorption, excitation, and luminescence spectra of aqueous solutions 1 mg/ml of nucleotides, their components, and mixtures with mannitol (in ratio 1:4). We observed a change in the ratio between the peaks of the spectra of acidic and salt forms of nucleotides.
Conclusions. The observations confirmed that nucleotides, nucleosides, and nucleic acid bases exhibit luminescence at room temperature, which might be useful information for further research in this area. In addition, a comparative analysis of the spectra showed possible interactions between nucleotide molecules and mannitol.
Key words. Nucleic acids, luminescence, mannitol. pyrimidines.
REFERENCES
1. Nauta M., Peeters D. J., Van Doorslaer T. F. S., Badkar A. V., Darvari R., Warne N. W., Jean J.,
Hendrikse D. P. G., Sahin U., Güler A., Kuhn A., Muik A., Vogel A., Walzer K., Witzel S., Hein S.,
Türeci Ö. Coronavirus vaccine. US Patent PCT/EP2021/060004. April 16, 2021.
2. Snitserova O. M. Terent’yeva Y.G., Pogrebna A.V., Koval Ju. V., Yashchuk V. M. The perspectives
of using the luminescent methods in studying biological tissues for medical application. Bulletin
of Taras Shevchenko National University of Kyiv Series Physics & Mathematics 2013, 4: 275-280
3. Terent’yeva Y.G., Snitserova O.M. Room temperature fluorescence of adenosin. Bulletin of Taras
Shevchenko National University of Kyiv. 2018, 1: 118–119
4. Gryn D.V., Yashchuk V.M., Tkachuk Z.Yu. Some peculiarities of spectral properties of various
compounds of adenine nucleotide and polynucleotide. Molecular Crystals and Liquid Crystals.
2021, 717(1): 54–59. https://doi.org/10.1080/15421406.2020.1860528
5. Yashchuk V.M., Kudrya V.Yu. The spectral properties of DNA and RNA macromolecules at low
temperatures: fundamental and applied aspects. Methods Appl. Fluoresc. 2017, 5: 014001. https://
doi.org/10.1088/2050-6120/aa50c9
6. Yashchuk V. M., Losytskyy M.Yu, Lebedyeva I. V., Ohulchanskyy T. Y., Piryatynskyy Yu P.,
Navozenko O. M. & Vretik L.O. Some peculiarities of electronic and vibronic excitations transfer
in organic media and hybrid nanosystems. Molecular Crystals and Liquid Crystals. 2020, 696(1):
3–14. https://doi.org/10.1080/15421406.2020.1731075
7. Borresen H.C. On the luminescence properties of some purines and pyrimidines. Acta
ChemicaScandinavica. 1963, 17(4): 921–929 https://doi.org/10.3891/acta.chem.scand.17-0921
8. Tkachuk Z. Yu. Multiantivirus compound, composition and method for treatment of virus diseases.
US Patent 8,420,617,B2. April 16, 2013.
9. Yashchuk V.M., Kudrya V.Yu., Levchenko S.M., Tkachuk Z.Yu., Hovorun D.M., Mel’nik V.I.,
Vorobyov V.P., Klishevich G.V. Optical Response of the Polynucleotides-Proteins Interaction.
Molecular Crystals and Liquid Crystals. 2011, 535 (1): 93–110. https://doi.org/10.1080/1542140.2
011.537953
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2024