ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 17, No. 2 , 2024
P. 36-37, Bibliography 9, Engl.
UDC::57.08+543.4
DOI: https://doi.org/10.15407/biotech17.02.036
ABSORPTION AND LUMINESCENCE PROPERTIES OF ACID AND SALT FORMS OF MONONUCLEOTIDES, THEIR COMPONENTS AND COMPLEXES WITH D-MANNITOL AT ROOM TEMPERATURE
M.A. Dotsenko1, R.O. Nikolaiev1, Z.YU. Tkachuk1
1Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv
Aim. This work aimed to analyze and compare spectral properties of aqueous nucleotide solutions in conditions close to biological systems. We studied the absorption and luminescence (Ex and Em fluorescence and Em phosphorescence) of monoribonucleotides, their disodium salts, bases, and nucleosides, and mixed them with D-mannitol dissolved in water at room temperature.
Methods. There were measured absorbance spectra using a Specord 210plus instrument and fluorescence excitation and emission and phosphorescence spectra using Horiba Fluoro Max 4+ instruments.
Results. There were obtained the absorption, excitation, and luminescence spectra of aqueous solutions 1 mg/ml of nucleotides, their components, and mixtures with mannitol (in ratio 1:4). We observed a change in the ratio between the peaks of the spectra of acidic and salt forms of nucleotides.
Conclusions. The observations confirmed that nucleotides, nucleosides, and nucleic acid bases exhibit luminescence at room temperature, which might be helpful information for further research in this area. In addition, a comparative analysis of the spectra showed possible interactions between nucleotide molecules and mannitol.
Key words. Nucleic acids, luminescence, mannitol. pyrimidines.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2024