ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 17, No. 1 , 2024
P. 20-28, Bibliography 16, Engl.
UDC:: 608:576
DOI: https;//doi.org/10.15407/biotech17.01.020
Full text: (PDF, in English)
DIRECTIONS OF DEVELOPING NEW TECHNOLOGIES BASED ON CULTIVATION OF ANIMAL CELLS AND TISSUES
T.M. Lutsenko, A. S. Chernetsky, P.S. Kovalchuk, A.V. Prozor, O.O. Krailo, B.A. Ruzhytska
National Technical University of Ukraine
“Igor Sikorsky Kyiv Polytechnic Institute”
Animal cell cultures found applications in various fields, from essential to advanced research. This includes studying the fundamentals of cell biology, mechanisms of the cell cycle, specialized cell functions, cell-cell and cell-matrix interactions, toxicity testing for the new drugs styling, and gene therapy for replacing non-functional genes with functional cells, characterization of cancer cells, understanding the role of various chemical substances, viruses, and radiation in cancer cells, vaccine production, monoclonal antibodies, and pharmaceuticals. Additionally, cultivating viruses for use in vaccine production, such as for diseases like rabies, hepatitis B, and measles, is another important application of animal cell cultures.
Aim. This study purposed to analyze the literature data on the use of animal cell lines in genetic engineering, therapy, xenotransplantation, biopharmaceuticals, the food industry, and research.
Methods. The analytical literature data review was conducted using the information analysis of the Medline (PubMed), Web of Science, and Scopus databases, Google Scholar, the Cochrane Central Register of Controlled Trials (CENTRAL), and other sources up to the inclusive year 2023 using the keywords: “animal cell lines”, “immunobiological preparations”, “xenotransplantation”, “biopharmaceuticals”, “genetic engineering”.
Results. The research analysis related to the animal cells used in the biopharmaceutical industry was carried out, and considerations regarding the prospects for their use in various research and production technologies were outlined.
Conclusion. The technology of cultivating animal cells became a fundamental tool in developing research in biotechnological sciences. The ability to culture animal cells in vitro allowed the development of innovative methods, such as iPSC and organ-on-a-chip models, which provided valuable information about the disease mechanisms and potential therapeutic targets. Although there are some challenges with the use of animal cells related to variability in differentiation efficiency and concerns about safety and efficacy, further studies are needed to optimize protocols and overcome these limitations. Animal cell culture technology remains essential to modern biomedical research and can potentially revolutionize regenerative medicine..
Key words: animal cell lines, immunobiological preparations, xenotransplantation, biopharmaceuticals, genetic engineering.
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© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2024