2022 №1(en)

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SSN 2410-7751 (Print)
ISSN 2410-776X (Online)

iotechnologia Acta Т. 15, No. 1, 2022
P. 72-80. Bibliography 22, Engl.
UDC: 579.6:604
https://doi.org/10.15407/biotech15.01.072

BIOTECHNOLOGICAL ASPECTS OF THE DEVELOPMENT OF A LIQUID FORMULATION OF MULTIFUNCTIONAL ENZYBIOTIC ANTISEPTIC

T. S. Todosiichuk ¹, O. V. Pokas ², S. О. Soloviov ³, T. S. Ryzhkova ¹

¹Igor Sikorsky Kyiv Polytechnic Institute, Kyiv
²SI “L.V. Gromashevsky Institute of Epidemiology and Infectious Diseases of NAMS of Ukraine”, Kyiv
³ Shupyk National Healthcare University of Ukraine, Kyiv

Aim. Evaluation of biotechnological aspects of the production of a liquid formulation of the multifunctional antiseptic preparation of microbial origin, which can be typified as an enzybiotic; characterization of the enzyme specificity of the studied formulation, stabilization methods, its ability to affect microbial biofilms.

Methods. Gel-filtration and electrophoresis were used to study the component composition and the specificity of the enzyme complex of the Streptomyces albus UN 44 producer strain. Proteolytic and staphylolytic activities of individual fractions were determined. The Pseudomonas aeruginosa biofilm and its formation under the effect of various drug formulations were quantitatively evaluated by spectrophotometry.

Results. The stability of the liquid formulation of the enzybiotic biosynthesized by S. albus UN 44 was demonstrated. Activity of the formulation could be prolonged and additionally stabilized by adding of 0.5% polyvinyl alcohol. Fractionation of the formulation enzyme complex using gel-filtration revealed the presence of at least three proteinases of different molecular weights (80-100, 24-35 and 20 kDa) and lysoenzymes (18-22 kDa). The effectiveness of the developed liquid antibiotic formulation for the destruction and inhibition (8-10 folds) of the biofilm formation by clinical strains of P. aeruginosa was shown.

Conclusions. The broad spectrum, multidirectional mechanisms of antimicrobial and regenerative action of enzybiotic drug, and the possibility of its production directly from the biotechnological process determine the prospects of its manufacturing and use as a multifunctional surface antiseptic.

Key words: antibiotic, antimicrobial action, Streptomyces albus, liquid formulation, biofilms, pathogens.

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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta Т. 15, No. 1, 2022
P. 61-71. Bibliography 47, Engl.
UDC: 547.915
https://doi.org/10.15407/biotech15.01.061

CYTOTOXIC, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF Peganum harmala L. EXTRACTS

Dehiri Mounira ¹, Diafat Abdelouahab¹, Fatmi Widad ², Ben Mansour Riadh ³, Bouaziz Farid ¹, Bahloul Ahmed ²

¹Laboratory of Characterization and Valorization of Natural Products, Faculty of Nature and Life Sciences, University El-Bachir El-Ibrahimi, Bord jBou Arreridj
² Laboratory of Health and Environment, Faculty of Nature and Life Sciences, University El-Bachir El-Ibrahimi, Bord jBou Arreridj
³Laboratory Analysis, Valuation of Food Securities, Biotechnology Research Group and Pathologies, National School of Engineering of Sfax (ENIS), Tunisia

Peganum harmala L., known as ?Harmel?, is a plant widely used in the traditional Algerian medicine.

Aim. The purpose of this work is to study the antioxidant, antiproliferative and antimicrobial potential of Peganum harmala extracts.

Methods. Colorimetric methods were used to quantify phenolic compounds, while the antioxidant activity was estimated in vitro using DPPH/ABTS radical scavenging assay, ferric reducing power, ?-carotene bleaching assay, total antioxidant capacity, and ferrous iron chelating assay. The agar well diffusion and the broth microdilution method were used to evaluate the antibacterial activity and the MTT assay was used to test the cytotoxicity of the extracts.

Results. The ethanolic extracts of Peganum harmala L. showed the highest polyphenols content and the potent antioxidant, gave a good activity against Gram + and Gram- bacteria and good antifungal effect and were more cytotoxic to the HeLa cell line.

Conclusions. It is concluded that selected plants could be a potential source of bioactive compounds with antioxidant, antimicrobial and antiproliferative potential. Hence, it is indicated to further investigate this plant in vitro as well as in vivo for new drug discovery.

Key words: Peganum harmala. polyphenols, antioxidant activity, antimicrobial effect, cytotoxic effect.

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ISSN 2410-776X (Online)

Biotechnologia Acta Т. 15, No. 1, 2022
P. 52-60. Bibliography 31, Engl.
UDC: 616.12-005.8:577.175.82:612.82.015.38.08
https://doi.org/10.15407/biotech15.01.052

CARDIOPROTECTIVE EFFECT OF ENKEPHALINS UNDER IMMOBILIZATION STRESS

І. J. Shcheniavskyi

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Objective: The aim of this study was to investigate the cardioprotective effect of dalargin, a synthetic leu-enkephalin.

Methods: The induction of myocardial infarction in rats, which were kept on a diet with excess fat and calcium/sodium salts for two months, by the use of immobilization stress. The experimental results indicated that the applied model allowed to induce the development of myocardial infarction within one three days, which was confirmed by electrocardiography, enzyme-linked immunosorbent assay and histological examination.

Results: Pre-treatment of rats with dalargin had no prevented myocardial infarction, however, it increased the resistance to immobilization stress and reduced infarction-induced myocardial lesions. Simultaneous administration of naloxone, an opiate receptor antagonist, together with dalargin eliminated its cardioprotective effect in experimental animals.

Conclusion: The use of synthetic leu-enkephalin dalargin significantly reduced the risk of myocardial infarction caused by excessive neuromuscular stress. The dalargin effect on the myocardium was mediated by opiate receptors.

Key words: myocardial infarction, immobilization stress, dalargin.

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17. Perazzo J., Castanho M. A., S? Santos S. Pharmacological Potential of the Endogenous Dipeptide Kyotorphin and Selected Derivatives. Front. Pharmacol. 2017, V. 7, P. 530. https://doi.org/10.3389/fphar.2016.00530

18. Gulevsky O. K., Schenyavsky I. I. Antihypoxant Activity of Low Molecular Weight Fraction Bovine Blood Cryohemolysate at Different Stages of Ontogenesis. Problems of Cryobiology and Cryomedicin. 2017, 27 (1), 41–50. https://doi.org/10.15407/cryo27.01.041

19. Gulevsky A. K., Abakumowa E. S., Shenyavsky I. I. Biological activity of low molecular weight fraction obtained from cord and peripheral blood in cows of different ages. Fiziol. Zh. 2017, 63 (2), 73?79. https://doi.org/10.15407/fz63.02.073

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31. Shcheniavsky I. Anti-Apoptotic Effect of Synthetic Leu-Enkephalin Dalargin on Rat Leukocytes in Cold Stress Model in Vivo. Probl. Cryobiol. Cryomed. 2021, V. 31, P. 3–13. https://doi.org/10.15407/cryo31.01.003

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ISSN 2410-776X (Online)

Biotechnologia Acta Т. 15, No. 1, 2022
P. 43-51. Bibliography 29, Engl.
UDC: 615.214.22.015.11
https://doi.org/10.15407/biotech15.01.043

METABOLIC PROFILE AND MECHANISMS OF GABA-TARGETED RECEPTOR PROPOXAZEPAM METABOLIZATION IN HUMAN HEPATOCYTES

M. Golovenko ¹, A. Reder^2, V. Larionov ¹, S. Andronati ¹

¹Physico-Chemical Institute of the National Academy of Sciences of Ukraine, Odesa
² SLC «INTERCHEM», Odesa, Ukraine

The aim of this study was to identify the Propoxazepam metabolites, formed by suspension of cryopreserved human hepatocytes, using the precise method of mass LC-MS/MS analysis.

Methods. A suitable chromatographic method was developed for the profiling of Propoxazepam and its metabolites. Samples were analyzed using a Waters Vion high resolution LC-MS/MS instrument, and data were examined using Waters Unifi software to determine the identity of the most abundant metabolites. Following a 4-hour incubation with human hepatocytes, intact Propoxazepam molecule accounted for 96.0% of the profile. Its most abundant metabolite was the oxidize.

Results. Propoxazepam (3-hydroxyderivative), which accounted for approximately 2.5% of the total peak response in the 4-hour sample. Two minor components were also found, each accounting for < 10% of the total peak response. Glucuronic conjugates have not been found under the experimental conditions. All metabolites formed represented less than 10% of the total chromatographic peak response.

Coclusion. The data obtained indicate the absence of reactive electrophilic derivatives among the metabolites of Propoxazepam.

Key words: Propoxazepam, humanhepatocytes, metabolism, LC-MS/MS analysis.

References

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6. Golovenko M., Reder A., Andronati S., Larionov V. Evidence for the involvement of the GABA-ergic pathway in the anticonvulsant and antinociception activity of Propoxazepam in mice and rats. J. Pre-Clin. Clin. Res. 2019, 13 (3), 99?105. https://doi.org/10.26444/jpccr/110430

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Biotechnologia Acta Т. 15, No. 1, 2022
P. 23-42. Bibliography 125, Engl.
UDC: 519.8.612.007
https://doi.org/10.15407/biotech15.01.023

MATHEMATICAL MODELS OF HUMAN RESPIRATORY AND BLOOD CIRCULATORY SYSTEMS

N. I. Aralova ¹, O. M. Klyuchko ², V. I. Mashkin¹, I. V. Mashkina ³, P. A. Radziejowski ⁴, M. P. Radziejowski ⁵

¹V. M. Glushkov Institute of Cybernetics of the National Academy of Sciences of Ukraine, Kyiv
²National Aviation University Kyiv, Ukraine
³Boris Grinhenko Kiyv University, Ukraine
⁴Kazimiera Milanowska College of Education and Therapy , Poznan, Poland
⁵Czestochowa University of Technology, Czestochowa, Poland

Aim. To analyze modern approaches to mathematical modeling of human respiratory and blood circulatory systems.

Methods. Comprehensive review of scientific literature sources extracted from domestic and international resources databases.

Results. Historical information and modern data concerning mathematical modeling of human functional respiratory and blood circulatory systems were summarized and analyzed in present ¬review; current trends in approaches to the construction of these models were revealed.

Conclusions. Currently, two main approaches to the mathematical modeling of respiratory and blood circulatory systems exist. One of them is the construction of models of the mechanics of respiration and blood circulation. They are based on the models of mechanics of solid deformable body, thermomechanics, hydromechanics, and continuum mechanics. This approach uses complex mathematical apparatus, including Navier-Stokes equation, which makes it possible to obtain a number of theoretical results, but it is hardly usable for real problems solutions at present time. The second approach is based on the model of F. Grodins, who represented the process of breathing as a controlled dynamic system, described by ordinary differential equations, in which the process control is carried out according to the feedback principle. There is a significant number of modifications of this model, which made it possible to simulate various disturbing influences, such as physical activity, hypoxia and hyperemia, and to predict parameters characterizing functional respiratory system under these disturbing influences.

Key words: mathematical model of respiratory system, mathematical model of blood circulatory system, hypoxic state, theoretical analysis.

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ACETONE-BUTYL FERMENTATION PECULIARITIES OF THE BUTANOL STRAINS -PRODUCER O. O. Tigunova, V. V. Bratishko, A. V. Balabak, S. G. Priyomov, S. M. Shulga

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