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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)


Biotechnologia Acta  Т. 15, No. 1, 2022
P. 5-22. Bibliography, 113 Engl.
UDC: 579.222


O. O. Tigunova 1, V. V. Bratishko 2, A. V. Balabak 3, S. G. Priyomov 1, S. M. Shulga 1

1 SE "Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine", Kyiv
2 National University of Life and Environmental Science of Ukraine, Kyiv
3 Uman National University of Horticulture, Uman, Ukraine

The aim of this review was to generalize and analyze the features of acetone-butyl fermentation as a type of butyric acid fermentation in the process of obtaining butanol as an alternative biofuel.

Methods. The methods of analysis and generalization of analytical information and literature sources were used in the review. The results were obtained using the following methods such as microbiological (morphological properties of strains), chromatographic (determination of solvent concentration), spectrophotometric (determination of bacterial concentration), and molecular genetic (phylogenetic analysis of strains).

Results. The process of acetone-butyl fermentation was analyzed, the main producer strains were considered, the features of the relationship between alcohol formation and sporulation were described, the possibility of butanol obtaining from synthesis gas was shown, and the features of the industrial production of butanol were considered.

Conclusions. The features of the mechanism of acetone-butyl fermentation (the relationships between alcohol formation and sporulation, the duration of the acid-forming and alcohol-forming stages during batch fermentation depending on the change in the concentration of H2, CO, partial pressure, organic acids and mineral additives) and obtaining an enrichment culture during the production of butanol as an alternative fuel were shown. The possibility of using synthesis gas as a substrate for reducing atmospheric emissions during the fermentation process was shown. The direction of increasing the productivity of butanol-producing strains to create a competitive industrial biofuel technology was proposed.

Key words: producer strains, biofuel, biobutanol, acetone-butyl fermentation.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2021