ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta V. 14, No 2, 2021
Р. 28-36, Bibliography 24, English
Universal Decimal Classification: 612.617.1:615.014.41:539.21-022.532
https://doi.org/10.15407/biotech14.02.028
N. O Volkova, M. S. Yukhta, L. V. Sokil, L. G. Chernyschenko, L. V. Stepanyuk, A. M. Goltsev
Institute for Problems of Cryobiology and Cryomedicineof the National Academy of Sciences of Ukraine, Kharkiv
An optimal approach to the recovery of testicular tissue fragments after cryopreservation is critical for their further use in order to successful fertility restoration.
Aim. The purpose of this study was to investigate the effect of bovine serum albumin (BSA) addition to the rehabilitation medium on the morphofunctional characteristics of fragments of seminiferous tubules of testes (FSTT) of immature rats after cryopreservation.
Methods. The object of the study was cryopreserved by slow cooling and vitrified FSTT. Warmed samples were incubated for 30 min in Leibovitz medium supplemented with BSA at concentrations of 0, 2, 5 or 10%. After that, morphological characteristics, the activity of the metabolic and antioxidant systems were evaluated.
Results. It was found that in the samples cryopreserved by slow cooling, the use of 5% BSA contributed to the increase in the safety of spermatogenic epithelium cells, in the levels of metabolic and antioxidant activities. In case of vitrified FSTT samples, it was observed that the addition of 5% BSA to the medium promoted the repair of minor tissue damage and increased metabolic activity level, but did not affect the state of the antioxidant defense system.
Conclusions. The obtained data can be used to develop an effective rehabilitation medium for cryopreserved fragments of the seminiferous tubules of testes using BSA.
Key words: bovine serum albumin, rehabilitation, cryopreservation, fragments of the seminiferous tubules of testes.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2021
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