ASSESSMENT OF GENETIC STRUCTURE VARIABILITY OF RAINBOW TROUT Oncorhynchus mykiss (WALBAUM, 1792) OF UKRAINIAN LOCAL STOCKS USING POLYMORPHIC BLOOD PLASMA PROTEINS О. Yu. Bielikova, S. I. Тarasjuk, A. I. Mruk, Т. A. Nahorniuk, L. P. Buchatskyi

Details: Category: 2_2021(en); Hits: 217

ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta V. 14, No 2, 2021
Р. 37-46, Bibliography 34, English
Universal Decimal Classification: 575.17:639.371.13(477)
https://doi.org/10.15407/biotech14.02.037

ASSESSMENT OF GENETIC STRUCTURE VARIABILITY OF RAINBOW TROUT Oncorhynchus mykiss (WALBAUM, 1792) OF UKRAINIAN LOCAL STOCKS USING POLYMORPHIC BLOOD PLASMA PROTEINS

О. Yu. Bielikova, S. I. Тarasjuk, A. I. Mruk, Т. A. Nahorniuk, L. P. Buchatskyi

Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine, Kyiv

Rainbow trout is a valuable species of aquaculture, which is characterized by a high level of variability.

Aim. The goal was to study the peculiarities of genetic variability of rainbow trout based on polymorphism of transferrin (TF), posttransferrin (PTF), esterase (EST) (EC 3.1.1.1.) and albumin (ALB) loci.

Methods. Electrophoretic separation of plasma proteins of rainbow trout from three local stocks (Chernivtsi, Kharkiv and Transcarpathian) was carried out in the native polyacrylamide gel.

Results. Peculiarities of the distribution and relative electrophoretic mobility of allelic variants of the studied loci of rainbow trout of Ukrainian local stocks were established.The results demonstrated the effectiveness of the analysis using methods of biochemical genetics to establish the characteristics of the genetic structure, the level of heterozygosity of local stocks and to carry out their differentiation.

Conclusion. The use of selected markers would allow monitoring the dynamics of changes in the state of local stocks under the established conditions of cultivation in the future.

Key words: rainbow trout, polymorphic blood plasma proteins, transferrin, posttransferrin, esterase, albumin.

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