ISSN 410-7751 (Print)
ISSN 2410-776X (on-line)
"Biotechnologia Acta" V. 10, No 4, 2017
https://doi.org/10.15407/biotech10.04.044
Р. 44-52, Bibliography 22, English
Universal Decimal Classification: 606:546.289:662.7
I. A. Blaydа, N. Yu. Vasylieva, T. V. Vasylieva, L. I. Sliusarenko
Odesa National Mechnykov University, Ukraine
The aim of the work was to optimize the process of germanium bioleaching from the dumps after coal beneficiation, namely, to determine the optimal composition of the new nutrient medium for acidophilic chemolithotrophic bacteria ensuring the maximum recovery of valuable metal in minimum time. We optimized the method of mathematical planning adapted to the plan in Greek-Latin squares. The calculations in this approach are based on the analysis of variance. The formal design of experiments has been carried out with four operating factors at four levels. The calculations were performed in Excel. The significance of the factor levels were analyzed using the Duncan’s multiple range test, the uniformity of the variances was examined the Cochran test, and the significance of the factors was tested by the Fisher criterion for each day of the experiment. The obtained results were interpreted mathematically and biologically. The following combination of factors and their levels was recommended as optimum nutrient medium, g/dm3: KH2PO4 — 1.0; (NH4)2SO4 — 2.0; KCl — 0.1; MgSO4 — 0.5; NH4Cl — 0.5; Na2S2O3 — 5.0. The proposed composition allows the more than 90% quick extraction of germanium into the solution (in four days), which was previously impossible.
Key words: bioleaching, acidophilic chemolithotrophic bacteria, germanium, coal beneficiation, Greek-Latin squares, variance analysis.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2017
References
1. Blayda I., Vasyleva T., Slyusarenko L., Abisheva Z., Ivanytsia V. The germanium extraction from industrial wastes by microbiological methods. XXVI International Mineral Processing Congress (IMPC 2012): New Delhi, India. 2012, P. 550–558.
2. Blayda I. A., Vasileva T. V., Baranov V. I. The use of bio-hydrometallurgical technologies in solving problems utilization of manmade waste and receiving from them valuable metals. Kompleksnoe ispolzovanie mineralnogo syirya. 2015, V. 3, P. 75–82. (In Russian).
3. Blayda І. A., Vasileva T. V., Slyusarenko L. І., Khitrich V. F., Barba І. M., Іvanitcia V. O., Baranov V. І. The method of extracting rare metals from waste coal industry. UA Patent 102926. August 27, 2013. (In Ukrainian).
4. Blayda I. A., Vasyleva T. V., Baranov V. I., Slyusarenko L. I., Baklan V. Yu. Opportunities bacterial and chemical leaching of coal refuse with aim to germanium and gallium extraction. Izvestiya Vuzov. Prikladnaya khimiya i biotekhnologiya. 2013, 1 (4), 54–60. (In Russian).
5. Blayda I. A. The study of composition and activity of bacterial community of coal tailing. Biotechnol. acta. 2014, 7 (5), 94–100. https://doi.org/10.15407/biotech7.05.094
6. Blayda І. A., Vasileva T. V., Baranov V. I., Semenov K. I., Slyusarenko L. I., Barba I. N. Рroperties of chemolithotrophic bacteria new strains isolated from industrial substrates. Biotechnol. acta. 2015, 8 (6), 56–62. doi: 10.15407/biotech8. 06.056.
7. Vasylieva T. V., Blayda I. A., Sliusarenko L. I., Vasylieva N. Yu., Chitrich V. F. Bacterial leaching of the metals from waste flotation of coals with the participation of thiosulfate, ferrous and ferric iron. Microbiology & Biotechnology. 2016, V. 3, P. 43–56. https://doi.org/10.18524/2307-4663.2016.3(35).77956 (In Russian).
8. Karavayko G. I. Practical Guide to biogeotechnology metals. Moskva: AN SSSR. 1989, 371 p. (In Russian).
9. Methods for General Bacteriology. V. 2. Moskva: Mir. 1984, 265 p. (In Russian).
10. Grachev Ju. P., Plaksin Ju. M. Mathematical methods of experiment planning. Moskva: Nauka. 2005, 296 p. (In Russian).
11. Adler Ju. P., Markova E. V., Granovskij Ju. V. Planning an experiment when searching for optimal conditions. Moskva: Nauka. 1976, 280 p. (In Russian).
12. Zedginidze I. G. Planning an experiment to study multicomponent systems. Moskva: Nauka. 1976, 390 p. (In Russian).
13. Radchenko S. G. Analysis of experimental data based on the use of multifactorial statistical mathematical models. Matematichnі mashini і sistemi. 2005, V. 3, P. 102–115. (In Russian).
14. Ljubishhev A. A. Dispersion analysis in biology. Moskva: Izd-vo Mosk. un-ta. 1986, 200 p. (In Russian).
15. Tsarenko I. Yu., Roy A. O., Kurdish I. K. Оptimization of nutrient medium for cultivation of Bacillus subtilis IMV V–7023. Microbiol. J. 2011, 73 (2), 13–19. (In Russian).
16. Lengeler J., Drevs G. I., Shlegel' G. (Eds). Modern microbiology. Prokaryotes. V. 2. Moskva: Mir. 2005, 496 p. (In Russian).
17. Likesh I., Ljaga J. Basic tables of mathematical statistics. Moskva: Finansy i statistika. 1985, 365 p. (In Russian).
18. Lakin G. F. Biometrics. Moskva: Vysshaja shkola. 1990, 352 p. (In Russian).
19. Ahmed Risikat Nike, Ahmed Risikat Nike, Alafara A. Baba, Sani Al-Hasan, Alamu Folake Bosede, Ajijolakewu Abiodun Kamoldeen. Bioleaching of Lead from Nigerian Anglesite Ore by Acidithiobacillus ferrooxidans. J. Appl. Sci. Res. 2012, 12 (8), 5591–5598.
20. Sand W., Gehrke T., Jozsa P.-G., Schippers A. (Bio) chemistry of bacterial leaching – direct vs. indirect bioleaching. Hydrometallurgy. 2001, V. 59, P. 159–175.
21. Schippers A., Sand W. Bacterial leaching of metal sulfides proceeds by two indirect mechanisms via thiosulfate or via polysulfides and sulfur. Appl. Environm. Microbiol. 1999, 65 (1), 319–321.
22. Rodriguez Y., Ballester A., Blazquez M. L. New information on the pyrite bioleaching mechanism at low and high temperature. Hydrometallurgy. 2003, V. 71, P. 37–46.