"Biotechnologia Acta" V. 10, No 1, 2017
https://doi.org/10.15407/biotech10.01.007
Р. 7-16, Bibliography 25, English
Universal Decimal Classification: 579.841.1:57.063.8:632.95
E. A. Kiprianova1, L. A. Safronova1, A. O. Prosyanov2
1Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
2Ltd. Company “BіoNіk”, Mykolaiv
The aim of this review was to present the results of more than ten-year study of gaupsin biopreparation created on the basis of two strains Pseudomonas chlororaphis subsp. aureofaciens UCM В-111 and UCM В-306 with antifungal, entomopathogenic and antiviral activities. Data about antibiotic substances produced by these strains — phenazine and phenylpyrrole derivatives — are presented. Entomocidal properties against the wide spectrum of insect pests have been found out in the strains-producers. Antiviral activity of gaupsin due to the production of thermostable exopolymers containing neutral monosaccharides has been shown using the tobacco mosaic virus as a model. Lipopolysaccharides of the strains В-111 and В-306 also appeared to be highly active antiviral agents. Structure of their O-specific polysaccharides has been established. The last one are structurally heterogenic, presented by linear tri-and tetrasaccharide repeated links and have specific structure that has not been described previously.
Key words: gaupsin biopreparation, Pseudomonas chlororaphis subsp. aureofaciens, antibiotics – phenazines, pyrrolnitrine, entomopathogenic properties, antiviral activity.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2017
References
1. Chincholkar S., Tomashow L. Microbial Phenazines: Biosynthesis, Agriculture and Health. New York: Springer Berlin Heidelberg. 2013, 248 p. https://doi.org/10.1007/978-3-642-40573-0
2. Bernd H., Rehm A. Pseudomonas. Model Organism, Pathogen, Cell Factory. Wiley-VCH. 2008, 395 p.
3. Weller D. Pseudomonas Biocontrol Agents of Soil-born Pathogens: Looking Back over 30 years. Phytopathology. 2007, V. 97, P. 250–256. https://doi.org/10.1094/PHYTO-97-2-0250
4. Mercado-Blanco J., Bakker P. Interactions between plants and beneficial Pseudomonas spp.: exploiting bacterial traits for crop protection. Antonie van Leeuwenhoek. 2007, 92 (4), 367–89. https://doi.org/10.1007/s10482-007-9167-1
5. Tombolini R., Van der Gaag D., Gerhardson B., Jansson J. Colonization Pattern of the Biocontrol Strain Pseudomonas chlororaphis MA 342 on Barley Seeds Visualized by Using Green Fluorescent Protein. Applied and Environmental Microbiology. 1999, 65 (8), 3674–3680.
6. US Environmental Protection Agency. Pseudomonas chlororaphis strain 63-28 (006478) Fact Sheet. Cited by http://www.epa.gov/oppbppd1/biopesticides/ingredients/factsheets/factsheet_006478.
7. Kiprianova E., Goral S. Incektofungicidal preparation gaupsin for the control of pests and agricultural plants diseases. Patent of Ukraine № 73682 АО 1N 63/00 C 12 N 1/20, publ. 15. 08. 2005. (In Ukrainian).
8. C?zard C., Farvacques N., Sonnet P. (2015-01-01). Chemistry and biology of pyoverdines, Pseudomonas primary siderophores. Curr. Med. Chem. 2015, 22 (2), 165–186. https://doi.org/10.2174/0929867321666141011194624
9. Kiprianova E., Shepelevich V., Klochko V., Ostapchuk A., Varbanets L., Scoklyuk L., Berezkinas A., Avdeeva L. Antifungal and antiviral substances of Pseudomonas chlororaphis subsp. aureofaciens strains – components of gaupsin. Microbiol. Zh. 2013, 75 (6), 28–35. (In Russian).
10. Pierson L., Pierson E. Metabolism and function of phenazines in bacteria: impacts on the behavior of bacteria in the environment and biotechnological processes. Appl. Microbiol. Biotechnol. 2010, 86 (6), 1659–1670. https://doi.org/10.1007/s00253-010-2509-3
11. Wang D., Yu J. M., Dorosky R. J., Pierson L. S., Pierson E. A. The Phenazine 2-Hydroxy-phenazine-1-carbonic acid promotes extracellular DNA release and has broad transcriptonic consequences in Pseudomonas chlororaphis 30-84. PLoS One. 2016, 26, 11 (1), e0148003. https://doi.org/10.1371/journal.pone.0148003
12. Lee J., Ma K., Ko S., Kang B., Kim I., Kim Y. Nematicidal activity of a nonpathogenic biocontrol bacterium, Pseudomonas chlororaphis O6. Curr. Microbiol. 2011, 62 (3), 746–751. https://doi.org/10.1007/s00284-010-9779-y
13. Smirnov V., Kiprianova E. Bacteria of the Pseudomonas genus. Kyiv: Naukova dumka. 1990, 263 p. (In Russian).
14. Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F. Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila. Nat. Biotechnol. 2006, 24 (6), 673–679.
15. Xuemei S., Mingmin C., Hongbo H., Wei W., Huasong P., Ping X., Xuehong Z. Genome Sequence of Pseudomonas chlororaphis GP72, a Root-Colonizing Biocontrol Strain. J. Bacteriol. 2012, P. 1269–1270. https://doi.org/10.1128/JB.06713-11
16. Balko O., Kiprianova O., Коvalenko O., Shepelevitch V., Аvdeeva L. Antiphytoviral activity of biopreparation gaupsin. Microbiol. i Biotechnol. 2010, N 2, P. 51–57. (In Ukrainian).
17. Shepelevitch V., Shubchynskyy V., Varbanets L., Kiprianova E. Antiviral Activity of Carbohydrate-containing Biopolymers of Pseudomonas chlororaphis subsp. aureofaciens. Twenty-Fours International Conference on Antiviral Research (ICAR). Sofia, Bulgaria (May 8–11, 2011). 2011, Р. 64.
18. Kiprianova E., Varbanets L., Shepelevitch V., Voychuk S. Antiviral activity of Pseudomonas chlororaphis subsp. aureofaciens lipopolysaccharides. Biotechnologia Acta. 2013, 6 (2), 68–73. (In Russian).
19. Van Wees S., Van der Ent S., Pieterse C. Plant immune responses triggered by beneficial microbes. Curr. Opin. Plant. Biol. 2008, 11 (4), 443–448. https://doi.org/10.1016/j.pbi.2008.05.005
20. Varbanets L., Zdorovenko E., Kiprianova E., Avdeeva L., Brovarskaya O., Rybalko S. Characteristics of Pseudomonas chlororaphis lipopolysaccharides. Microbiology. 2015, 84 (6), 680–690. (In Russian).
21. Zdorovenko E., Varbanets L., Shaskov A., Kiprianova E., Knirel Y. Structure of the O-polysaccharide of the lipopolysaccharide of Pseudomonas chlororaphis subsp. aureofaciens UCM B-306. Carbohydr. Res. 2015, V. 410, P. 47–50. https://doi.org/10.1016/j.carres.2015.03.019
22. Zdorovenko E., Kadykova A., Varbanets D., Shashkov A., Kiprianova E., Brovarskaya O., Knirel Y. Structure of the O-specific polysaccharides of Pseudomonas chlororaphis subsp. chlororaphis UCM B-106. Carbohydr. Res. 2016, V. 433, P. 1–4. doi: org/10.1016/j.carres.2016.06.013.
23. Kiprianova E., Varbanets L., Rybalko S., Shepelevich V. Strain Pseudomonas chlororaphis subsp. aureofaciens producing lipopolysaccharide with antiviral activity. Patent of Ukraine № 76689, МPК 2013.01, С12N 1/00, publ. 10. 01. 2013. (In Ukrainian).
24. Varbanets L., Kiprianova E., Rybalko S. Strain Pseudomonas chlororaphis subsp. aureofaciens producing lipopolysaccharide with activity against viruses of influenza, herpes and hepatitis C. Patent of Ukraine № 103797, МPК 2015.01, С12N 7/00, publ. 25. 12. 2015. (In Ukrainian).
25. Shepelevich V., Kiprianova E., Yaroshenko L., Avdeeva L. Sensitivity of Pseudomonas chlororaphis strains to antibiotics and tools of plant protection. Microbiol. Zhurnal. 2012, 74 (6), 24–28 (In Russian).