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"Biotechnologia Acta" V. 10, No 1, 2017
https://doi.org/10.15407/biotech10.01.017
Р. 17-25, Bibliography 41, English
Universal Decimal Classification: 606:577.21

no1 2017

MOLECULAR MECHANISMS OF PLURIPOTENCY INDUCTION AND REPROGRAMMING OF SOMATIC CELLS

T. O. Deputatova

De Novo, LLC, Kyiv, Ukraine

To analyze the recent studies elucidating the molecular mechanisms of pluripotency induction and crucial stages of the reprogramming process was the aim of the review. The key focus is on the factors enabling switch between the reprogramming stages. It is concluded that one of the key barriers for iPSC applications is the multi-stage nature of somatic cells reprogramming that features both stochastic early phases and deterministic establishment of pluripotent regulatory network. Despite thousands of scientific studies, various reprogramming protocols restrict effective research analysis and identification of molecular reprogramming mechanisms. In order to specify accurate reprogramming algorithms and develop more effective protocols of patient specific reprogrammed cells cultivation, the future researches require focus on the phase transition switchers.

Key words: pluripotency induction, reprogramming, somatic cells, induced pluripotent stem cells.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2017