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"Biotechnologia Acta" V. 9, No 6, 2016
Р. 82-89, Bibliography 16, English
Universal Decimal Classification:  577.3.04

 6 2016


L. V. Marynchenko1, O. I. Nizhelska2, D. M. Lytvynenko1, G. M. Zabolotna3

1 National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute»
2The Scientific and Training Centre “Physical and Chemical Material Science”
3Institute of Food Biotechnology and Genomics of the National Academy of Sciences  of Ukraine

The aim of the research was the impact of non-thermal electromagnetic radiation of superhigh frequencies  with waves of millimeter range on threonine amino acid synthesis by bacteria Brevibacterium flavum for ordinary (not mutant) and mutant strains.

The frequencies of millimeter range waves were selected according to previous works as 41.76; 42.2 and 61.0 GHz. The exposition was 10 min. The control samples of bacterial suspension in the flasks were kept under the same conditions as the test ones. Irradiated suspensions were used as inoculum for fermentation on molasses wort at t = +30 ?C with aeration. After cultivation for 3 days the samples irradiated with frequency 42.2 and 61.0 GHz gave an increase in colonies forming units, respectively, 1.4 and 1.9 times compared to the control for the non-mutant strain. The quantity of synthesized threonine was determined by thin-layer chromatography on the plates of ciluprevir. A significant increase of the threonine content in the culture fluid was observed for the non-mutant strain (70% compared to control) after the irradiation with frequency 61.0 GHz. The splitting of the colonies planted pigmentation was observed: the control samples were mostly pigmentated, and irradiated bacteria lost this ability immediately after exposure, but after the culturing the irradiated samples restored pigmentation. The pigmentation ability was confirmed by the data on the accumulation threonine in the culture fluid.

The Brevibacterium flavum mutant strain did not respond to the irradiation, this influence was negative for generative abilities and accumulation of threonine in the culture fluid.

Ключевые слова: Brevibacterium flavum, threonine, nonthermal electromagnetic radiation of millimeter range waves.

© Институт биохимии им. А. В. Палладина НАН Украины, 2016