"Biotechnologia Acta" V. 9, No 6, 2016
https://doi.org/10.15407/biotech9.06.082
Р. 82-89, Bibliography 16, English
Universal Decimal Classification: 577.3.04
L. V. Marynchenko1, O. I. Nizhelska2, D. M. Lytvynenko1, G. M. Zabolotna3
1 National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute»
2The Scientific and Training Centre “Physical and Chemical Material Science”
3Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine
The aim of the research was the impact of non-thermal electromagnetic radiation of superhigh frequencies with waves of millimeter range on threonine amino acid synthesis by bacteria Brevibacterium flavum for ordinary (not mutant) and mutant strains.
The frequencies of millimeter range waves were selected according to previous works as 41.76; 42.2 and 61.0 GHz. The exposition was 10 min. The control samples of bacterial suspension in the flasks were kept under the same conditions as the test ones. Irradiated suspensions were used as inoculum for fermentation on molasses wort at t = +30 ?C with aeration. After cultivation for 3 days the samples irradiated with frequency 42.2 and 61.0 GHz gave an increase in colonies forming units, respectively, 1.4 and 1.9 times compared to the control for the non-mutant strain. The quantity of synthesized threonine was determined by thin-layer chromatography on the plates of ciluprevir. A significant increase of the threonine content in the culture fluid was observed for the non-mutant strain (70% compared to control) after the irradiation with frequency 61.0 GHz. The splitting of the colonies planted pigmentation was observed: the control samples were mostly pigmentated, and irradiated bacteria lost this ability immediately after exposure, but after the culturing the irradiated samples restored pigmentation. The pigmentation ability was confirmed by the data on the accumulation threonine in the culture fluid.
The Brevibacterium flavum mutant strain did not respond to the irradiation, this influence was negative for generative abilities and accumulation of threonine in the culture fluid.
Ключевые слова: Brevibacterium flavum, threonine, nonthermal electromagnetic radiation of millimeter range waves.
© Институт биохимии им. А. В. Палладина НАН Украины, 2016
References
1. Andriiash G. S., Zabolotna G. M., Tkachenko A. F., Blume Ya. B., Shulga S. M. Threonine synthesis of Brevibacterium flavum mutant strain. Threonine: Food Sources, Functions and Health Benefits. 2015, (Nova), P. 1–26.
2. Andriyash G. S., Zabolotna G. M., Shulga S. M. Auxotrophity of producents of lysine. Biotechnologiya. 2012, 5 (1), 70–77.
3. Andriyash G. S., Zabolotnа G. M., Shulga S. М. The mutant strains of microorganisms ? producers of lysine and threonine. Biotechnol. аcta. 2014, N 3, P. 95–101. https://doi.org/10.15407/biotech7.03.095
4. Shulga S. M., Tigunova O. O., Tkachenko A. F., Beyko N. E., Andriash G. S., Priemov S. G. Threonine biosynthesic intensification by Brevibacterium flavum ТН-7 strain. Biotechnologia. 2011, N 5, P. 97–103. (In Ukraine).
5. Grundler W., Keilmann F., Putterlik V., Santo L., Strube D., Zimmermann I. Nonthermal resonant effects of 42 GHz microwaves on the growth of yeast cultures. In: Frohlich H., Kremer F. (eds.) Coherent excitations in biological systems. Springer, Berlin Heidelberg New York. 1983, P.21–37. https://doi.org/10.1007/978-3-642-69186-7_4
6. AlipovYe. D., Belyaev I. Ya., Aizenberg O. A. Systemic reaction of Escherichia coli cells to weak electromagnetic fields of extremely low frequency. Bioelectrochem. Bioenerget. 1994, V. 34, P. 5–12. https://doi.org/10.1016/0302-4598(94)80002-2
7. Kalinichenko S. V. The influence of millimeter electromagnetic waves high frequency range on the adgesive properties of Corynebacteria. The Journal of V. N. Karazin Kharkiv National University. 2004, 9 (639), 7–11.
8. Webb S. J. Nonlinear Phenomenain Bioenergetics and Oncology as seen as 25 years of research with mm microwaves and Raman spectroscopy. Nonlinear Electromagnetics in Biological Systems. Ed. by W. R. Adey. New York, London, Plenum Press. 1984, 603 p.
9. Jankovi? S. M., Milo?ev M. Z., Novakovi? M. L. The Effects of Microwave Radiation on Microbial Culture Hospital Pharmacology. 2014, 1 (2), 102–108.
10. Marynchenko L. V., Nizhelska O. I., Marynchenko V. O. Stimulation of Accumulation ofBiomass and Fermenting Activity of Saccharomyces cerevisiae Yeast by Extra High Frequency Electromagnetic Irradiation. Research Bulletin of Ukraine Kyiv Polytechnic Institute. 2011, 3 (77), 68–73.
11. Marinchenko V. O., Nizhel’skaya O. I., Makara V. A., Yakunov A. V., Marinchenko L .V. Ukraine Inventor’s Certificate no. 102480, Byull. 2013, N 13, 4 p.
12. Banik S., Bandyopadhyay S., Gangulyal S. Bioeffects of microwave – a brief review. Bioresource Technology. 2003, V. 87, P. 155–159. https://doi.org/10.1016/S0960-8524(02)00169-4
13. Grundler W., Keilmann F. Sharp resonance in yeast growth prove nonthermal sensitivity in microwaves. Phys. Rev. Lett. 1983, V. 51, P. 1214–1216. https://doi.org/10.1103/PhysRevLett.51.1214
14. Yakunov A. V., Nizhelska A. I., Marinchenko L. V., Marinchenko V. A., Makara V. A. Influence of Processing of Yeast Saccharomyces cerevisiae with Millimeter Waves on Fermentation Indicesin Technology of Bioethanol Production. Surface Engineering and Applied Electrochemistry. 2015, 51 (2), 156–161. https://doi.org/10.3103/S1068375515020143
15. Andreev E. A., Belyi M. U., Ivanchenko I. A., Yakunov A. V. Determination of threshold power of electromagnetic field of millimeter range affecting thegrowth of yeast cells. Elektron. Obrab. Mater. 1990, N 1, P. 61–63.
16. Marinchenko V. O., Nizhel’skaya O. I., Makara V. A., Yakunov A. V., Marinchenko L. V. Ukraine Inventor’s Certificate no. 102905, Byull. 2013, N 16, 4 p.