ISSN 2410-7751 (Print)
ISSN 2410-776X (on-line)
"Biotechnologia Acta" V. 9, No 3, 2016
https://doi.org/10.15407/biotech9.03.052
Р. 52-60 , Bibliography 21, English
Universal Decimal Classification: 541.49:546.732/3:547.496.26577.152.34:577.152.32
1 Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
2 Mechnikov Odesa National University, Ukraine
The aim of the research was to study the effect of a number of coordination compounds of stanum and germanium (compounds 1?8) as modifiers of activity of peptidases and ?-galactosidases. The coordination compounds with the same type structure of were investigated as enzymes effectors. Two types of complexes: 1) [M(H2O)6][Ge(НCitr)2]•4H2O (M = Mg(1), Mn(2), Co(3), Ni(4), Zn(5)), containing biscitrate-stanate anion ([Ge(НCitr)2]2-), and 2) [M(H2O)6][Sn(НCitr)2]•4H2O (M = Mg(6), Co(7), Ni(8)), containing biscitratostanate anion and various hexaaquacations ([M(H2O)6]2+, М= Mg, Mn, Co, Ni, Zn) were studied. It is shown that the compound 6 which is a biscitratostanate complex containing magnesium ions as metal, can be used for stimulation on 20–25% of collagenase activity of B. thuringiensis var. israelensis IMV B-7465 peptidase 1 and peptidase 2. Compound 1 (biscitrate-stanate complex containing magnesium ions as metal) and 7 (biscitratostanate complex containing cobalt ions as metal) and compound 6 in a concentration of 0.001% are able to increase elastolytic activity of peptidase 1 on 55–58%. However compound 7 has shown the greatest activating effect. It increased the elastolytic activity of peptidase 2 on 100–140% (for both tested concentrations). This indicates that the compound 7 can further be used as of peptidase 2 elastolytic activity effector. In the study of effect of the considered coordination compounds on the activity of α-galactosidase of Penicillium canescens, Cladosporium cladosporioides and Aspergillus niger was found that when using a number of complexes (1–2 and 4–8), there is a slight increase on 12–20% of enzyme activity of P. canescens, and the maximum effect (~20%, concentration 0.01%) was provided by complex 6.
Key words: B. thuringiensis var. israelensis, ИМВ В-7465 peptidases, α-galactosidases, biscitrate-stanate and biscitratostanate complexes.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2016
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