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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

3 2016

"Biotechnologia Acta" V. 9, No 3, 2016
Р. 44-51, Bibliography 38, English
Universal Decimal Classification: 577.122:577.112.083
https://doi.org/10.15407/biotech9.03.044

INTERACTION OF RECOMBINANT DIPHTHERIA TOXOIDS WITH CELLULAR RECEPTORS in vitro

 K. Yu. Manoilov, A. Ju. Labyntsev, N. V. Korotkevych, D. V. Kolibo, S. V. Komisarenko

Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kyiv

The aim of the research was to compare in vitro characteristics of reception of the natural diphtheria toxin — DT and its nontoxic recombinant analogs — toxoids. For assessing ligand-receptor interaction the method of immunoenzyme analysis and ELISA was used, where the bonding layer recombinant analogues of diphtheria toxin cell receptor HB-EGF from sensitive and resistant to the toxin of the organisms were served. According to the results of ELISA the natural diphtheria toxin, in contrast to recombinant toxoids — CRM197, and B subunit, interacted with mouse HB-EGF with a very low affinity. While human HB-EGF with an equally high affinity connected as toxoids as native diphtheria toxin. Therefore, the analyzed recombinant analogs of toxin obtained in E. coli cells did not reproduce in full measure the receptor specificity of the natural toxin, which should be considered in the case of using these proteins as biotech products.

Key words: diphtheria toxin, B subunit of diphtheria toxin, CRM197, HB-EGF.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2016