ISSN 2410-7751 (Print)
ISSN 2410-776X (on-line)
Ж-л "Biotechnologia Acta" Т. 9, № 2, 2016
https://doi.org/10.15407/biotech9.02.070
P. 70--81, Bibliography. 21, English.
UDC: 57.08:636:31
POSSIBILITY OF COMPUTER EXPERIMENT IN STUDY OF ANIMAL SPERMATOZOA HETEROGENEITY
L. V. Gorbunov, Y. M. Mazharova
National Technical University «Kharkiv Polytechnic Institute», Ukraine
A simulation model for evaluating the survival and fertilizing capacity of animal spermatozoa was developed, taking into account the initial condition of the sperm and the effectiveness of cryopreservation stages. The model is based on an analytical expression that reflects the main reasons for the survival of reproductive cells in onto-, techno- and phylogenesis. The decrease in spermatozoa resistance depends on a number of biological factors — the animal species, physiological conditions of sperm donor and recipient, the ejaculate quality, and technological factors — the effectiveness of the methods of cell cryopreservation and egg insemination. The discrepancy between the results of cell motility obtained by calculation and experimental methods amounted to less than 2% as a result of our own experiments and to less than 5% for the data taken from literature. A feature of the model is the complete independence of the effectiveness of studied techniques from the heterogeneity of animal sperm.
The conducted computer experiment showed that the difference between the values of initial motility and fertilizing capacity of sperm varies from 50 to 100% depending on the difference of biological parameters, while the index of the effectiveness of selected technique creates an error of about 1%. Comparative analysis of alternative technologies of spermatozoa cryopreservation showed the maximum efficiency of the stages of cryoprotectant use, freeze mode, survival and fertilizing capacity of the object. The use of computer modeling allows to greatly reduce the spread in spermatozoa preservation values that were obtained in different experiments, and thus to reduce the time and costs it takes to obtain reliable results.
Key words: system biology, cryopreservation, heterogeneity, spermatozoa.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2016
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