ISSN 2410-7751 (Print)
ISSN 2410-776X (on-line)
"Biotechnologia Acta" V. 9, No 2, 2016
https://doi.org/10.15407/biotech9.02.082
Р. 82-86, Bibliography 17, English
Universal Decimal Classification: 602.6:635.9
NUTRIENT MEDIA FOR OBTAINING A PURE CULTURE OF FUNGI OF THE GENUS Pleurotus in vitro
1 National University of Life and Environmental Sciences of Ukraine, Kyiv
2 National University of "Kyiv-Mohyla Academy", Ukraine
3Kyiv Regional State Administration
The aim of the study was modification of the nutrient medium for basidiomycetes of the genus Pleurotus and its linear velocity of the growth determination. Biotechnology (obtaining and subcultivation of the strain NC-35 in vitro) and statistical methods were used. It was found that previously known media do not provide sufficient speed of mycelium growth and necessary biomass yield. There were investigated the new components of nutrient medium for greater biomass of pure culture of higher basidiomycetes of the genus Pleurotus obtaining. The nutrient medium for pure cultures of higher basidiomycetes, which contains agar-agar and water, additionally included the oat grain and oak bark decoctions in the following ratio per 1 l: agar-agar 15 g, oat grain decoction 600 ml, oak bark decoction 250 ml, distilled water — up to 1 l. The results of the linear growth rate analysis of Pleurotus ostreatus on the test and the control nutrient media, the calculation of the correlation coefficient indicate that for isolation and cultivation of oyster mushroom strains the most suitable medium is medium with the content of oat grain and oak bark decoctions. The obtained results will be used in the future for pure cultures of fungi of the genus Pleurotus obtaining in vitro.
Key words: optimized culture medium, basidiomycetes of the genus Pleurotus.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2016
References
1. Morozov A. I. Cultivation of mushrooms. Mycelium. ACT. 2007, 43 р. (In Russian).
2. Becker Z. E. Physiology and biochemistry of fungi. Moskwa: MGU. 1988, 230 p. (In Russian).
3. Alekseeva K. L. Cultivated mushrooms. Scientific and production reference. Publ Ros. Agricultural. Academy. Moskwa: Izd-vo Ros. Sel'khozakademii. RASKHN. 2000, 223 p. (In Russian).
4. Belitskii I. V. Sowing the mycelium of edible and medicinal mushrooms ksylotrophic: technology of cultivation and quality criteria. Gavrysh. 2000, P. 11. (In Ukrainian).
5. Babitskaya V. G. Pleurotus ostreatus producing a complex of biologically active substances. Moskwa: Prikladnaya biokhimiya i mikrobiologiya. 1996, V. 32,
P. 203–210. (In Russian).
6. Bisko N. A. Biology and cultivation of edible mushrooms. Kуiv: Naukova dumka. 1987, 148 p. (In Russіan).
7. Bisko N. A. An integrated approach to the cultivation of oyster mushrooms Kуiv. 2001, 54 p. (In Russian).
8. Dudka A. A., Bisko N. A., Bilai V. G. Cultivation of edible mushrooms. Kуiv: Urozhaі. 1992, 160 p. (In Russian).
9. Bisko N. A. The microflora of the substrate Pleurotus ostreatus. Mikologiya i fitopatologiya. 1996, V. 30, P. 7–12. (In Russian).
10. Becker M. E. Introduction to biotechnology. Riga: Pishchevaya promyshlennost'. 1978, 231 p. (In Russian).
11. Sychov P. A., Nehrutskii S. F., Tkachenko N. P., Timofeev A. A.,
Kaliberda G. V. Pat. # 29725 A Ukraina. A breeding ground for use with pure cultures of higher Basidiomycetes – cultivation of industrial. Zayavka № 97020763 vid 02/21/97, 6 A01G1/04, C12N1/14. (In Ukrainian).
12. Sychov P. A., Timofeev A. A., Kaliberda G. V., Hardzhala L. I.,
Zahumennyi R. A. Pat. # 29744 A Ukraine. Growth media to update the collection of strains of fungi of the genus oyster. Zayavka № 97041764 vid 15.04.97 6 A01G1/04, C12N1/14. (In Ukrainian).
13. Bisko N. A. The edible medicinal mushroom oyster mushroom. Ekolohiia dovk?llia ta bezpeka zhittediialnosti. 2008, V. 3, P. 61–65. (In Russian).
14. Vdovenko S. A. Cultivation of edible mushrooms: training manual. V?nnytsya: RVV. 2011, P. 49–52. (In Ukrainian).
15. Golub G. A., Abrosimov G. L., Gaidenko A. I., Tomashchuk A. I. Technological process of of the substrate for growing oyster. Naukovyi svit. 2010,
30 p. (In Ukrainian).
16. Ha Thi Hoal, Chun-Li Wang. The Effects of Temperature and Nutritional Conditions on Mycelium Growth of Two Oyster Mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology. 2015, V. 43, P. 14–23.