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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

 6 2015

"Biotechnologia Acta" V. 8, No 6, 2015
https://doi.org./10.15407/biotech8.06.063
Р. 63-70, Bibliography 22, English
Universal Decimal Classification: 611.018.5.013.8.088.3:577.121.7:57.43

ENERGY METABOLISM OF PACKED WHITE CELLS AFTER CRYOPRESERVATION AND REHABILITATION IN A MEDIUM CONTAINING A CORD BLOOD LOW-MOLECULAR FRACTION

A. K. Gulevsky1, Yu. S. Akhatova1, А. А. Sysoev2, I. V. Sysoeva2

1Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkiv
2The A.O. Kovalevsky Institute of Biology of the Southern Seas of National Academy of Sciences of Ukraine, Sevastopol

To study the bioenergy indicators of leykokontsentrate cells after cryopreservation and the possibility of their recovery after incubation in a medium with a low-molecular fraction from cow cord blood were the aim of research. Leykokontsentrate was obtained from donor blood by sedimentation; cryopreservation was carried in the slow freezing regimen with 5% dimethylacetamide; amount of ATP, ADP and AMP was determined by chemiluminescence; glycogen — by cytochemical method. Evidence of energy disbalance of leukoconcentrate cells after cryopreservation was obtained. It was shown that the cord blood low-molecular fraction (0.15 mg/ml) activated glycogenolysis and increased contents of ATP, ADP and AMP in leukoconcentrate cells after cryopreservation. It was also shown that the cord blood low-molecular fraction contained energy substrates and metabolites, hormones and macro- and micronutrients. Use of the low-molecular fraction (below 5 kDa) from cord blood as a component of rehabilitating medium for frozen-thawed leukoconcentrate cells contributed to improvement of their energy status, which was manifested as augmentation in the total adenylic pool and glycogenolysis activation.

Key words: leykokontsentrat, cryopreservation, energy metabolism, cord blood.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2015