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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

6 2015

"Biotechnologia Acta" V. 8, No 6, 2015
Р. 56-62, Bibliography 15, English
Universal Decimal Classification: 579:662.7


I. A. Blayda1, T. V. Vasyleva1, V. I. Baranov2, K. I. Semenov1, L. I. Slysarenko, I. M. Barba1

1 Mechnykov Odesa National University, Ukraine
2 Franko Lviv National University, Ukraine

The purpose of the research was determination of strains Acidithiobacillus ferrooxidans MFLv37 and Acidithiobacillus ferrooxidans MFLad27, isolated from aboriginal consortium of coal beneficiation dumps and fly ash from coal combustion, resistance to heavy metals, forming part of these waste, as well as adaption ability of the strains to new substrates. New strains increased resistance to heavy metal ions as compared to A. ferrooxidans standard and collection strains is found; minimal inhibitory concentrations of heavy and toxic metals are determined; a number of metals that have negative impact on growth of isolated cultures are identified. It is shown that the minimal metals concentrations, at which strains growth still happens, are several times higher than their concentrations in technogenic waste. It has been established that isolated strains differed in their ability to adapt, as well as in growth rate and substrates oxidation. This is due to the specific conditions of microbiocenoses formation in making and further storage of rock dumps and fly ash, whereof the appropriate strains are isolated. The investigations indicate the necessity in directional selection of strains that are resistant to the toxic compounds and are able to oxidize various mineral substrates, as well as in their adaptation to new substrates for the extraction of heavy metals.

Key words: fly ash, rock dumps, aboriginal bacterial community, acidophilic chemolithotrophic bacteria, strains, leaching activity, ions of heavy metals.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2015