ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)ФФ
"Biotechnologia Acta" v. 6, no. 5, 2013
https://doi.org/10.15407/biotech6.05.108
Р.108-114, Bibliography 15, Ukrainian
Universal Decimal classification: 577.336
Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkiv
2State Scientific Institution «Institute for Single Crystals of the National Academy of Sciences of Ukraine», Kharkiv
The work aimed to investigate the use of new probes Е-176, К8-3000 and К8-1300 to research the specificity of peptides in the extracts of cryopreserved fragments of organs of pigs’ and piglets’ and their interaction with blood serum albumin. Extracts of cryopreserved fragments of skin of new-born piglets’, spleen of pigs’, heart of mature pigs’ and new-born piglets’ cleared of proteins were used in the research.
Titration of extracts with investigated probes revealed differences in the peptides properties that are a part of them, to bind probes, reflecting tissue specificity of peptide drugs.
Adding the extracts to albumin solution triggered quenching of intrinsic fluorescence of albumin and affected the state of tryptophan residues of the protein.
Investigated probes show a high sensitivity to the interaction with blood serum albumin substances of peptide nature and could be recommended as new fluorescent probes for use in the biomedical researches. Since fluorescence of these probes is excited in the long wavelength of spectrum, it enables to reduce the artifacts by their use that are introduced by chromophore groups of the biomacromolecules absorbing in the visible region.
Key words: fluorescent probes, extracts of organs, peptides, albumin.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2013
References
- Gavrilov V. B. Defining the parameters of the binding of a fluorescent probe pirronovogo red with human serum albumin. Biofizika. 2001, 46 (1), 39–42. (In Russian).
- Albani J. R. Relation between Proteins tertiary structure, tryptophan fluorescence lifetimes and tryptophan So ? 1Lb and So ? 1La transitions. Studies on a1-acid glycoprotein and b-lactoglobulin. Fluoresc. 2011, 21 (3), 1301–1309.
https://doi.org/10.1007/s10895-010-0813-z - Hawe A., Friess W., Sutter M., Jiskoot W. Extrinsic fluorescent dyes as tools for protein characterization. Res. 2008, 25 (7), 1487–1499.
https://doi.org/10.1007/s11095-007-9516-9 - Klitgaard S., Neves Petersen M. T., Petersen S. B. Quenchers induce wavelength dependence on protein fluorescence lifetimes. Fluoresc. 2006, 16 (4), 595–609.
https://doi.org/10.1007/s10895-006-0081-0 - Wang B., Li H. W., Gao Y. A multifunctional fluorescence probe for the detection of cations in aqueous solution: the versatility of probes based on peptides. Fluoresc. 2011, 21 (5), 1921–1931.
https://doi.org/10.1007/s10895-011-0891-6 - Tatarets A. L., Pavrozin E. A., Dyubko T. SS. New long-fluorescent probes for studying proteins. rep. XXI Intern. scientific and practical. conf. "Application of lasers in medicine and biology", Odessa, 26–29 May 2004. P. 134. (In Russian).
- Dyubko T. S., Okladnoy Yu. G. On the approaches to new fluorescent probes for properties and biomembranes structural changes research selection and classification. 8th Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes (MAFS). Prague, Czech Republic, August 24–27, 2003. 208.
- Galchenko S. E. Storage of water-solovih ekstraktіv krіokonservovanih fragmentіv organіv pigs. kriobiol. 2004, N 4, P. 72–78. (In Ukrainian).
- Galchenko S. E. Extracts of cryopreserved fragments ksenoorhan preparation and biological effect. kriobiol. 2005, 15 (3), 403–406. (In Ukrainian).
10. Galchenko S. E., Dyubko T. S., Patsenker L. D., Sandomirskiy B. P. Spectrofluorimetric study of extracts of cryopreserved fragments of pigs. I. intrinsic fluorescence. Eksper. klin. med. 2006, N 1, P. 48–52. (In Russian).
11. Galchenko S. E., Дюбко Т. С., Sandomirskiy B. P., Patsenker L. D. Spectrofluorimetric study extracts of cryopreserved fragments of pigs. II. Analysis using fluorescent probe К-35. Eksperym. klin. med. 2006, N 3, P. 57–61. (In Russian).
12. Pat. 64381 А Ukraine, MPK7 А61К35/12. Method of obtaining of xenogeneic extracts. S. E. Galchenko, N. Yu. Shkodovska, B. P. Sandomirskiy, V. I. Hrishchenko. IPKiK NAN Ukrainy. № 2003054649; Appl. 22.05.2003; Publ. 16.02.2004, Bul. Promysl. vlasnist, N 2. (In Ukrainian).
13. Gavrilov V. B. Characteristics of sensitivity and specificity of the structural state of the fluorescent markers and protein binding capacity. Biofizika. 2000, 45 (3), 421–426. (In Russian).
14. Serum albumin in clinical medicine. Edd. Yu. A. Gryzunov and G. E. Dobretsov. Мoskva: IRIUS. 1994, 226 p. (In Russian).
15. Serum albumin in clinical medicine. Edd. Yu. A. Gryzunov and G. E. Dobretsov. Book 2. Мoskva: GEOTAR. 1998, 440 p. (In Russian).