ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)
"Biotechnologia Acta" v. 6, no. 5, 2013
https://doi.org/10.15407/biotech6.05.100
Р.100-107, Bibliography 21, Ukrainian
Universal Decimal classification: 576.32/36; 57.085.23
EXPRESSION OF PLURIPOTENCY MARKERS IN REPROGRAMMING WITH TRANSPOSON SYSTEM MURINE FIBROBLASTS
S. V. Malysheva1, 2, N. M. Bilko1, J. Hescheler2
1National University of «Kyiv-Mohylanska Academy», Kyiv, Ukraine
2Institute of Neurophysiology, University of Cologne, Cologne, Germany
The search for effective and safe methods to generate induced pluripotent stem cells is especially urgent. In the paper murine embryonic fibro blasts were reprogrammed towards actively proliferating colonies with typical induced pluripotent stem cells morphology by means of Sleeping beauty transposon-based vector system. The obtained clones were checked for the expression of various pluripotency markers: alkaline phosphatase, Oct4 and Sox2 genes, SSEA-1 expression in various clones was evaluated. Also the reactivation of endogenous pluripotency factors Nanog and Rex1 was indicated. The data obtained is analyzed and compared to the established pluripotent stem cell line. It is shown that somatic cells are reprogrammed towards pluripotency by means of Sleeping beauty transposon system. Therefore, the system is a new perspective biotechnological tool to generate pluripotent cells.
Key words: induced pluripotent stem cells, reprogramming, pluripotency markers.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2013
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