ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 6, no. 1, 2013
https://doi.org/ 10.15407/biotech6.01.097
Р. 97-104, Bibliography 10, Ukrainian.
Universal Decimal classification: 579.222
NEW PRODUCER STRAINS OF BIOBUTANOL.
І. ISOLATION AND IDENTIFICATION
SO «Institute of Food Biotechnology and Genomics» of National Academy of Sciences of Ukraine, Kyiv
Getting new, more productive strains of microorganisms that produce butanol is a topical problem. Studing of morphological and physiological characteristics of the isolated strains, improvement of their cultivation conditions, optimization of biobutanol synthesis gives the possibility to organize a cost-effective butanol production technology. The aim of this work was searching new butanol and butyric acid producer strains, their identification and studying the main steps of the selective strains biosynthesis. The objects of this study were microorganisms that had allocated from soils and sludges samples of Kiev’s lakes. Obtained cultures have been screened. Three strains were obtained as promising and identified as C. acetobutylicum, C. tyrobutylicum, C. butylicum. Selective medium have been developed and modified for the microorganisms. Producer’s features were investigated in order to maximize the accumulation of target metabolites.
Key words: biobutanol, biosynthesis, ABE-fermentation, producer strains.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008
References
1. Tigunova O., Shulga S. Obtaining of new butanol producers. Abst. 15thEur. Congr.Biotechnol., Istanbul, Turkey, 23–29 September 2012. V. 29, Issue S. P. S 43.
2.International patent US 5 753 474. Continuous two stage, dual path anaerobic fermentation of butanol and other organic solvents using two different strains of bacteria. D. E. Ramey. Filed 20.12.1996; Published 19.05.1998.
3. Biobutanol: history, technology manufacturers. Internet magazine "Commercial Biotechnology". Internet journal “Kommercheskaya biotekhnolohiia”. 2009. Available at http://www.cbio.ru/article.php?sto-ryid=3355.
4. Ren Z., Ward T. E., Logan B. E., Regan J. M. Characterization of the cellulolytic and hydrogen-producing activities of six mesophilic Clostridium species. J. Appl. Microbiol. 2007, 103 (6), 2258–2266. (In Russian).
5. Berezina O. V. Cellulase and hemicellulase activity of solventogennyh clostridia. Collection of the articles "Biotechnology of the future" at the International Symposium "EU - Russia: prospects for cooperation in the field of biotechnology in the 7th Framework Programme". M.: OAO “Aviaizdat”, 2006, P. 4–5. (In Russian).
6. Yastrenskaia L. S., Vasylchenko O. A. Selection of anaerobic cellulolytic thermophilic strain Clostrіdіum thermo -cellum 5CT. Biotekhnolohiia. 2011, 4(2), P. 80–85. (In Russian).
7.Guillaume Bruant, Marie-Josee levesque et. al. Genomic Analysis of Carbone Monoxide Utilization and Butanol Production by Clostridium carboxidivorans Stain p7T. Plose ONE. 2010, 5(9), P. 1–12.8.
8. Short determinant of Bergi bacteria. Ed. Dzh. Khoult. M.: Mir. 1980, 495 p.
9. Sliusarenko T. P. Laboratory workshop on food microbiology production. Book third, revised and enlarged. M.: Legkaia I pishchevaia promyshlennoct. 1984, 208 p. (In Russian).
10. Enterobacteriaceae. Guidelines for doctors. Ed. V. I. Poplavskii. M.: Meditsina. 1985, 317 p. (In Russian).