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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
1 2013

"Biotechnologia Acta" v. 6, no. 1, 2013 10.15407/biotech6.01.081
Р. 81-85, Bibliography 13, Ukrainian.
Universal Decimal classification: 546.284+577.112


T. M. Chernyshenko, V. I. Gryshchuk, N. P. Galagan, V. O. Chernyshenko,
O. V. Gornytska, T. M. Platonova, E. V. Lugovskoy

Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv
Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine, Kyiv

Using of high-dispersed silica based sorbents is a new and prospective method of bleeding arrest. Studies of high-dispersed silica haemostatic action during superficial wounds were promising for its surgery usage.

However the mechanisms of haemostatic effects of high-dispersed silica are still unknown. Our investigations were focused on high-dispersed silica action on activation of some blood coagulation factors and clearing of its blood arresting mechanisms.

Activation of individual coagulation factors was measured using specific chromogenic substrates. It was shown that high-dispersed silica increases the activation of intrinsic and extrinsic blood coagulation pathway factors which was
initiated by «Activated partial thromboplastin time» and thromboplastin. Blood coagulation factor X was determined as a target of high-dispersed silica using highspecific activators from the snake venoms. Observed increasing of factor X activation could be the consequence of the adsorption of the intrinsic blood coagulation pathway factors on sorbent surface.

Obtained results give a possibility to speculate about high-dispersed silica effects on blood coagulation increasing.

Key words: high-dispersed silica, hemostasis system, factor Х.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008