ISSN 1995-5537

«Біотехнологія» journal V. 5, No. 6, 2012
Р. 78-84, Bibliography 17, Russian.
Universal Decimal classification: 576.8:620.193

STUDY OF THE BIOFILM EXOPOLIMERYC COMPLEX STRUCTURE FORMED BY CORROSIVE BACTERIA BIOFILM

M. O. Boretska, O. S. Suslova, V. F. Gorchev, S. A. Karahim

Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv
Ukrainian National Center «Institute of Biology» Kyiv National Taras Shevchenko University
Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv

Carbohydrate and protein components of the biofilm formed corrosion active bacteria Thiobacillus thioparus 224M and heterotrophic satellite of Stenotrophomonas maltopilia 22M on the mild steel surface were studied. It was found that the cocultivation leads to appear of monosaccharides not previously encountered in the matrix of monocultures (rhamnose, ribose, xylose, galactose). By confocal laser scanning microscope LSM 510 META (Carl Zaiss, Germany) it was studied the spatial localization of components of the biofilm matrix. Using fluorescently labeled lectin Concanavalin A (Sigma, USA) and dye binding with protein Thiazine Red (Sigma, USA) allowed to show the structural features of biofilm-related spatial localization of components. The obtained data for studying of the structure and function of biofilms are important for understanding the mechanisms of natural biofilms, medicine and respective industries.

Key words: biofilm, exopolymeric complex, fluorescence, sulfur cycle bacteria.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008