ISSN 1995-5537
«Біотехнологія» journal V. 5, No. 6, 2012
Р. 85-94, Bibliography 33, Ukrainian.
Universal Decimal classification: 577.152.321+663.11
Donetsk National University, Donetsk
Nutrient medium composition on sources of carbon and nitrogen for maximal activity of basidiomycetes cellulolytic enzymes has been optimized. It was found that among carbon sources the most pronounced effect on repressing the cellulases activity exhibits glucose. While being cultivated on nutrient mediums with disaccharides, basidiomycetes produced nonspecific cellobiase more than endoglucanase. To receive cultural liquids of basidiomycetes with highest total cellulolytic activity, strains should be cultivated on nutrient mediums containing filter paper as single carbon source in concentration 8 g/l. As a result of culture medium optimization for nitrogen source it was revealed that nitrites and urea display maximal inhibiting effect and maximuminduction occurs when nitrates are brought into the culture medium. The best nitrogen source for cellulases activity for К-1 Irpex lacteus strain is 1,5 g/l calcium nitrate; А-Дон-02 and Д-1 Irpex lacteus are 1,5 g/l ammonium nitrate; and AnSc-1 Daedaleopsis confragosa f. confragosa — 1,65 g/l ammonium sulfate. Optimal ratios of carbon to nitrogen for cellulases activity are 5,3:1 for К-1, А-Дон-02, Д-1 I. lacteus strains and 4,8:1 for AnSc-1 D. confragosa f. confragosa one.
Key words: basidiomycetes, cellulolytic enzymes, nutrient medium composition, carbon, nitrogen, Irpex lacteus, Daedaleo psis confragosa f. confragosa.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008