ISSN 1995-5537
«Біотехнологія», V. 5, No 6, 2012
P. 101-106, refer. 9, Ukrainian.
UDC 57.08:636:31
ANALYSIS OF THE REPRODUCIBILITY RESULTS
OF ANIMALS EMBRYOS СRYOPRESERVATION
Institute of Animal Husbandry of National Academy of Agrarian Sciences, Kharkіv
Ukrainian Academy of Agrarian Sciences, Kyiv
Technologies of the artificial fertilization and transplantation of сryopreservated sperm and embryos are the foundation of the breeding and selection of animals.
The comparative analysis is carried out, theoretical generalization and the new solution of increase of reproducibility of results of experiment on the basis of the offered mathematical model of the estimation of a native condition of embryos of cows and efficiency of stages of their сryopreservation are given. Application of model repeatedly increases reproducibility of results and provides the condition to their comparability when using different ways of freezing of embryos of cows. Experimental test of the offered model showed that use of the generalized factors of viability and efficiency reduces the dispersion of results to size of an error of their detection. The design values of the generalized values were as follows: viability of embryos of cows, which depended on physiological condition of their donors — 97.1±1.0% and recipients — 95.1±3.9%; efficiency of сryopreservation — 96.0±4.2% and transplantation — 54.3±4.8%.
The quantitative accounting of a condition of native bioobject, during determination of efficiency of stages of the different ways of the freeze, raised manifold the reproducibility of results of its сryopreservation, that allowed to reduce considerably the volume of the biological material and costs for carrying out the tests under condition of obtaining of reliable value.
Key words: reproducibility, efficiency of сryopre servation, embryos of a cow, safety of bioobject, viability.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008