ISSN 1995-5537

«Біотехнологія» journal V. 5, No. 5, 2012
Р. 109-113, Bibliography 16, Ukrainian.
Universal Decimal classification: 577.3:621.384.8:547.495.9

MASS-SPECTROMETRY STUDIES OF OLIGOMERES COMPOSITION
OF POLYHEXAMETHYLENEGUANIDINE

V. Lysytsya, A. V. Rebriev, V. V. Polischuk

Agriculture Institute of West Polissya of National Academy of Agrarian Sciences of Ukraine, Rivne
Palladian Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv

The results of mass-spectrometry studies of oligomere composition of polyhexamethyleneguanidine salts are given. Although polymer derivatives of guanidine are commonly applied as disinfectants, they are also capable to increase cell proliferation activity, regulate growth of microorganisms in biotechnology and stimulate germination of agriculture seeds. We have defined a construction of four types of oligomeres for polyhexamethyleneguanidine linear structure. They differ in composition of final groups. The identification, by means of mass-spectrometry, composition and constructions of the different sample polyhexamethyleneguanidine will hereinafter allow understanding the mechanisms of their biological activity. The variants of the mass-spectrometry method MALDI-TOF MS and TOF-PDMS were used. We have found that anion composition of polyhexamethyleneguanidine salts has a vital importance when ionizing in PDMS and little effect upon quality of the mass-spectra got by MALDI method.

Key words: polyhexamethyleneguanidine, oligomeres, mass-spectrometry method.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008